ELEISA KIT ya Trypsin

Recombinant Trypsin imagwiritsidwa ntchito nthawi zambiri popanga biopharmaceutical-panthawi yokonzekera ma cell kapena kusintha ndi kuyambitsa zinthu.Trypsin imakhala ndi ziwopsezo zachitetezo chifukwa chake iyenera kuchotsedwa musanatulutsidwe komaliza.Sandwich Kit iyi ndi yoyezera kuchuluka kwa Residual Trypsin mu cell culture supernatant ndi njira zina popanga biopharmaceutical trypsin ikagwiritsidwa ntchito.

Chida ichi ndi Enzyme-Linked Immunosorbent Assay (ELISA).Mbaleyi idakutidwa kale ndi Porcine trypsin antibody.Trypsin yomwe ilipo mu zitsanzo imawonjezedwa ndikumangirira ku ma antibodies omwe amakutidwa pazitsime.Kenako biotinylated Porcine trypsin antibody imawonjezedwa ndikumanga ku trypsin mu zitsanzo.Pambuyo kutsuka, HRP-Streptavidin imawonjezedwa ndikumangirira ku antibody ya trypsin ya Biotinylated.Pambuyo pa makulitsidwe, HRP-Streptavidin imatsukidwa.Kenako yankho la TMB gawo lapansi limawonjezedwa ndikupangidwa ndi HRP kuti apange mtundu wamtundu wabuluu womwe unasintha kukhala wachikasu pambuyo powonjezera acidic stop solution.Kuchulukana kwachikasu kumayenderana ndi kuchuluka kwa trypsin komwe mukufuna
chitsanzo chojambulidwa mu mbale.Kutsekemera kumayesedwa pa 450 nm.