Recombinant Trypsin
Ubwino
1.Palibe chiyambi cha nyama
2.Kuyera kwapamwamba
3.Kuwongolera njira yabwino
4.Stable khalidwe
Kufotokozera
Trypsin imadula makamaka zomangira za C-terminal peptide za lysine ndi arginine, zomwe zimatha kusokoneza mapuloteni omangira ma cell.Ma amino acid a trypsin ophatikizananso opangidwa ndi Hzymes Biotech ndi ofanana ndi a porcine pancreas-derived trypsin, ndipo amapangidwa ndi mawu ophatikizanso a Escherichia coli.Itha kulowa m'malo amtundu wa trypsin pakugayidwa kwa ma cell mu katemera, ma cell stem, ma cell cell therapy, kuyesa mankhwala, ma antibodies ndi magawo ena.Aprotinin, soya trypsin inhibitor, ndi zina zotero.
Recombinant trypsin ili ndi zinthu zofanana ndi trypsin yotengedwa mwachilengedwe.Chogulitsachi chimapangidwa m'makalasi a GMP.Palibe zida zoyambira zanyama zomwe zimagwiritsidwa ntchito pakuwotchera, kuyeretsa ndi zosakaniza zomaliza.Panthawi imodzimodziyo, njira zopangira ndi kuwongolera khalidwe zimatsimikiziridwa, zomwe zimapangitsa kuti pakhale mgwirizano wapamwamba pakati pa magulu.
Kapangidwe ka Chemical
Tanthauzo la Chigawo
25 ℃, pH 7.6, 3.2ml reaction solution (1cm kuwala njira), chigawo chimodzi cha trypsin (U) chinafotokozedwa ngati
kuwonjezeka kwa 0.003 mumtengo woyamwa pa 253nm ndi enzymatic hydrolysis ya BAEE pamphindi.
Kugwiritsa Ntchito Mankhwala
Za chikhalidwe cha ma cell
• Kutsekeka kwa minyewa yam'mimba, kutenga ma cell oyambira.
• Kudutsa m'mimba kwa maselo otsatizana.
• Chikhalidwe cha ma cell ndi njira ya microcarrier.
• Pang'onopang'ono kugaya maselo a tsinde.
• Chithandizo cha chitetezo cha mthupi, ndi zina zotero.
Kwa recombinant mapuloteni
• Kupanganso kwa insulin.
• Kutsatizana kwa mapuloteni, mapu a peptide.
• Njira zodziwika bwino za proteinolytic monga kafukufuku wa proteinomics
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Maonekedwe | White kapena wopanda ufa woyera |
| Kusungunuka (10mg/ml) | Chotsani njira yopanda mtundu |
| Chiyero | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Zochitika Zapadera (Ufa) | ≥2500U/mg |
| E.Coli zotsalira zama protein | ≤0.01% |
| Endotoxins(LAL-Mayeso) | ≤20 EU/mg |
Mayendedwe ndi kusunga
Mayendedwe:Kutumizidwa pansi pa 2-8 ° C
Posungira:Sungani pa -2-8 ° C, mutatha kusungunuka ndi 1mM hcl kapena 50nM HAC, iyenera kusungidwa pansi -25 ~ -15 °C
Moyo woyesereranso wovomerezeka:2 chaka
