Deoxyribonuclease I (Dnase I)
Kufotokozera
DNase I (Deoxyribonuclease I) ndi endodeoxyribonuclease yomwe imatha kugaya DNA imodzi kapena iwiri.Imazindikira ndikudula zomangira za phosphodiester kuti ipange monodeoxynucleotides kapena oligodeoxynucleotides yokhala ndi nsonga imodzi kapena iwiri yokhala ndi magulu a phosphate pa 5'-terminal ndi hydroxyl pa 3'-terminal.Ntchito ya DNase I imadalira Ca 2+ ndipo imatha kuyendetsedwa ndi ayoni achitsulo a divalent monga Mn 2+ ndi Zn 2+.5 mM Ca 2+ imateteza enzyme ku hydrolysis.Mg 2+ ikakhalapo, puloteniyo inkatha kuzindikira mwachisawawa ndi kuswa malo aliwonse pa chingwe chilichonse cha DNA.Pamaso pa Mn 2+, zingwe ziwiri za DNA zimatha kuzindikirika nthawi imodzi ndikumangika pafupi ndi malo omwewo kuti apange zidutswa za DNA kapena zidutswa zomata za DNA zomwe zili ndi 1-2 nucleotides.
Kapangidwe ka Chemical
Tanthauzo la Chigawo
Gawo limodzi limatanthauzidwa ngati kuchuluka kwa enzyme yomwe ingawononge 1 µg ya pBR322 DNA mu mphindi 10 pa 37 ° C.
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Chiyero (SDS-PAGE) | ≥ 95% |
| Ntchito ya Rnase | Palibe Kuwonongeka |
| Kuwonongeka kwa gDNA | ≤ 1 kopi/μL |
Mayendedwe ndi kusunga
Mayendedwe:Kutumizidwa pansi pa 0 °C
Posungira:Sungani kutentha kwa -25-15 ° C
Moyo woyesereranso wovomerezeka:2 chaka
