BsaI Restriction Endonuclease

Kuyera: 20U/μL

Phukusi: 50μL, 250μL, 1ml, 10ml, 100ml

Tsatanetsatane wa Zamalonda

Kufotokozera

The BsaI restriction enzyme ndi mtundu wa II restriction endonuclease wotengedwa ku protein recombinant yosungidwa ndi jini ya BspQI mu Bacillus sphaericus yofotokozedwa ndi E.coli.Ma endonucleases oletsa amagwiritsidwa ntchito kwambiri popanga mapu amtundu, cloning ndi madera ena.Enzyme iyi imadula mwachangu DNA kuti ipangitse bwino jini Bsa I, IIs restriction endonuclease restriction endonuclease, imachokera ku mtundu wina wa E. coli womwe umanyamula jini ya BsaI yopangidwa ndi kusinthidwa ndi kusinthidwa kuchokera ku Bacillus stearothermophilus.omwe ali ndi mawonekedwe ofanana ndi ma enzymes achilengedwe komanso kuchepa kwa nyenyezi.Kuzindikirika kwake ndi masamba ophatikizika ndi awa:

5'······GGTC(N)·········3'

3'······CCAGAG(NNNNN)······5'

Kapangidwe ka Chemical

Zogulitsa

• Choletsa endonuclease chimakhala ndi ntchito yayikulu ya enzymatic ndipo chimamaliza chimbudzi mwachangu.

• Ntchito yotsika yopanda endonuclease yotsika kuti muwonetsetse kudula "scalpel" molondola.

• Palibe BSA dongosolo, kuchepetsa kutentha gwero kuipitsa.

Kufotokozera

Zinthu Zoyesa	Zofotokozera
Ntchito ya enzyme	20 U/μL
Chiyero(SDS-PAGE)	≥95%
Rnase	Palibe chomwe chapezeka
Ntchito Zosagwirizana ndi DNase	Zosazindikirika
Nyenyezi Zochita	Zosazindikirika
Ligation ndi kudula	Pambuyo pa chimbudzi, zidutswa za DNA zimatha kulumikizidwa.Ndipo zidutswa za ligated izi zitha kudulidwanso ndi Bsal.
Endotoxins(LAL-test)	≤10 EU/mg
E.Coli DNA	≤1 Kope/2U

Kuwongolera khalidwe

Chiyero ≥ 95%, Palibe DNase, ntchito ya RNase, zotsalira za Host DNA ≤ 100pg/mg, zotsalira za mapuloteni ≤ -50ppm, zotsalira za endotoxin ≤ 10EU/mg, Palibe ntchito ya protease, yosabala, yopanda mycoplasma

Kugwiritsa ntchito

Digey the plasmid kukonzekera mzere wa DNA fragment kumapeto kwa Poly (A/T/C/G);

Lembani template ya plasmid musanayambe kulembedwa mu vitro;

Kuletsa digest;

Tanthauzo la Chigawo

Chigawo chimodzi chimatanthauzidwa ngati kuchuluka kwa enzyme yofunikira kuti igaye 1 µg ya Internal control DNA mu ola la 1 pa 37 ° C mu voliyumu yonse ya 50 µL.