Alkaline Phosphatase (ALP)

Alkaline Phosphatase amachokera ku mtundu wina wa E. coli womwe umanyamula jini ya TAB5.Enzyme imayambitsa dephosphorylation ya 5' ndi 3' kumapeto kwa DNA ndi RNA phosphomonoesters.Komanso, imatulutsa ribose, komanso deoxyribonucleoside triphosphates (NTPs ndi dNTPs).TAB5 Alkaline Phosphatase imagwira ntchito pa 5' yotuluka, 5' yotsalira komanso yosasinthika.Phosphatase imatha kugwiritsidwa ntchito pazinthu zambiri zama cell biology, monga kupanga cloning kapena probe end label kuchotsa ma phosphorylated a DNA kapena RNA.Pakuyesa kwa cloning, dephosphorylation imalepheretsa DNA ya plasmid yokhazikika kuti isadzipangire.Itha kutsitsanso ma dNTP osaphatikizidwa mu machitidwe a PCR kuti akonze template yotsatizana ndi DNA.Enzymeyi imakhala yosasunthika komanso yosasinthika potentha pa 70 ° C kwa mphindi zisanu, motero kumapangitsa kuti phosphatase ichotsedwe musanayambe kulumikiza kapena kuletsa kulemba zilembo kukhala zosafunikira.