Ultra Nuclease Assay Kit (ELISA)

Cas No.: 9025-65-4

EC.No.: 3.1.30.2

Phukusi: 96 Mayeso

Tsatanetsatane wa Zamalonda

Kufotokozera

Izi zida za Ultra Nuclease ELISA ndi mchenga womwe ELISA uyenera kuchitidwa mumtundu wa microplate.Zitsanzo zomwe zingakhale ndi endonuclease zimayikidwa mu zitsime za mbale za microtiter zomwe zidakutidwa kale ndi anti-endonuclease capture antibody yoyeretsedwa.Pambuyo pa kukulitsidwa ndi sitepe yotsuka yomwe zigawo zosamangidwa zimachotsedwa, anti-anti-endonuclease detector antibody imawonjezeredwa.Mphamvu ya immunological imapangitsa kuti pakhale sandwich complex ya solid phase antibody-endonuclease-enzyme yotchedwa antibody.Pambuyo pomaliza kutsuka, njira yapansi panthaka imawonjezeredwa ku zitsime ndikuchitapo kanthu, zomwe zimabweretsa chitukuko cha mtundu.Kuchuluka kwa kuwala kumayesedwa ndi photometrically ndipo kumayenderana ndi
ndende ya analyte yomwe ilipo m'zitsime.Kuchuluka kwa endonuclease m'zitsanzo zosadziwika kumatha kuwerengedwa kutengera njira yofananira.

Kapangidwe ka Chemical

Tanthauzo la Chigawo

Kuchuluka kwa enzyme yomwe imagwiritsidwa ntchito kusintha mayamwidwe a △A260 ndi 1.0 mkati mwa 30min
pa 37 °C, pH 8.0, yofanana ndi 37μg ya umuna wa salimoni yosungunuka mwa kudula mu oligonucleotides, imatanthauzidwa ngati gawo logwira ntchito.

Kugwiritsa Ntchito ndi Mlingo

• Chotsani exogenous nucleic acid ku mankhwala a katemera, kuchepetsa chiopsezo cha nucleic acid yotsalira kawopsedwe ndi kukonza chitetezo cha mankhwala.
• Chepetsani kukhuthala kwa madzi a chakudya chifukwa cha nucleic acid, kufupikitsa nthawi yokonza ndikuwonjezera zokolola.
• Chotsani nucleic acid yomwe idakulungidwa (virus, inclusion body, etc.), yomwe imathandizira kutulutsidwa ndi kuyeretsa kwa tinthu.
• Chithandizo cha nyukiliya chikhoza kupititsa patsogolo kuthetsa ndi kubwezeretsanso chitsanzo cha column chromatography, electrophoresis ndi blotting analysis.
• Mu chithandizo cha majini, nucleic acid imachotsedwa kuti ipeze mavairasi oyeretsedwa a adeno.