T4 RNA ligase
Kufotokozera
Izi ndi T4 RNA ligase yodalira ATP (T4 RNA Ligase I) yofotokozedwanso ndi E. coli.Enzyme iyi imathandizira kupanga zomangira za phosphodiester pakati pa oligonucleotides, RNA yokhala ndi chingwe chimodzi ndi DNA intermolecular / intra-molecular 5'-PO4 ndi 3'-OH.
Tanthauzo la Chigawo
Chigawo chimodzi chimatanthauzidwa ngati kuchuluka kwa enzyme yofunikira kuti asinthe nanomole imodzi ya 5'-[³²P] rA16 kukhala mawonekedwe osamva phosphatase mu mphindi 30 pa 37°C.
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Ntchito ya enzyme | 10 U/μL |
| Mapuloteni Ntchito | ≥ 95% |
| Nickase | Osazindikirika |
| Ntchito ya RNase | Osazindikirika |
| Ecoli gDNA | ≤1kopi/50U |
| Endotoxin | LCL-Mayeso,≤10EU/mg |
Mayendedwe ndi kusunga
Mayendedwe:Owuma ayezi
Posungira:Sungani pa -25 ~ 15 ° C (peŵani kuzizira mobwerezabwereza ndi kusungunuka)
Moyo woyesereranso wovomerezeka:2 chaka
zokhudzana ndi mankhwala
Lembani uthenga wanu apa ndikutumiza kwa ife
