Khwerero Chimodzi RT-qPCR Probe Kit-Kuzindikira kwa Mamolekyulu Owonetsedwa

Gawo limodzi la RT-qPCR probe kit

Phukusi: 100rxns, 1000rxns, 5000rxns

Tsatanetsatane wa Zamalonda

Kufotokozera

One Step qRT-PCR Probe Kit idapangidwira qPCR yomwe imagwiritsa ntchito RNA mwachindunji (monga virus RNA) ngati template.Pogwiritsa ntchito ma gene specific primers (GSP), zolembera m'mbuyo ndi qPCR zitha kumalizidwa mu chubu chimodzi, kuchepetsa kwambiri njira zamapaipi komanso chiwopsezo cha kuipitsidwa.Itha kuyimitsidwa pa 55 ℃ popanda kukhudza mphamvu ndi chidwi cha qRT-PCR.Kuphatikizira magwiridwe antchito apamwamba a HiScript III Reverse Transcriptase ndi Champagne Taq DNA Polymerase yoyambira yotentha, yokhala ndi makina okhathamira, mphamvu yozindikira ya One Step qRT-PCR Probe Kit imatha kufikira 0.1 pg ya RNA yonse kapena makope osakwana 10 a RNA templates. ndipo imakhala ndi kukhazikika kwamafuta apamwamba.Gawo limodzi la qRT-PCR Probe Kit limaperekedwa mu Master Mix.The 5 × One Step Mix ili ndi chosungira chokongoletsedwa bwino ndi dNTP/dUTP Mix, ndipo ndi yoyenera pazidziwitso zapamwamba kwambiri potengera ma probe olembedwa ndi fluorescence (monga TaqMan).

Njira Yochitira

Zigawo

Zigawo	100rx pa	1,000rns	5,000 rxns
RNase-free ddH2O	2 * 1 ml	20 ml pa	100 ml
5 * kusakaniza kwa sitepe imodzi	600μl	6*1ml pa	30 ml pa
Kusakaniza kwa enzyme imodzi	150μl	2 * 750μl	7.5ml pa
50 * ROX reference Dye 1	60μl pa	600μl	3 * 1 ml
50 * ROX reference Dye 2	60μl pa	600μl	3 * 1 ml