Proteinase K (Zamadzimadzi)
Ubwino wake
● Kukhazikika kwapamwamba ndi ntchito ya ma enzyme potengera umisiri wokhazikika wa chisinthiko
● Kusamva mchere wa Guanidine
● RNase yaulere, DNase yaulere ndi Nickase yaulere, DNA <5 pg/mg
Kufotokozera
Proteinase K ndi serine protease yokhazikika yokhala ndi gawo lalikulu la gawo lapansi.Imawononga mapuloteni ambiri m'dziko lakwawo ngakhale pamaso pa zotsukira.Umboni wochokera ku maphunziro a kristalo ndi mamolekyu akuwonetsa kuti enzymeyi ndi ya banja la subtilisin lomwe lili ndi tsamba lothandizira la triad (Asp 39-His 69-Ser 224).Malo odziwika kwambiri a cleavage ndi chomangira cha peptide choyandikana ndi gulu la carboxyl la aliphatic ndi zonunkhira za amino acid okhala ndi magulu otsekedwa a alpha amino.Nthawi zambiri amagwiritsidwa ntchito pazambiri zake.
Kapangidwe ka mankhwala
Kufotokozera
| Zinthu zoyesa | Zofotokozera |
| Maonekedwe (Mtundu) | Zopanda Mtundu mpaka Kuwala Brown |
| Maonekedwe (Turbidity) | Zowonekera Kwambiri Pang'ono Kwambiri |
| Mawonekedwe (Mawonekedwe) | Madzi |
| Zochita | ≥800U/ml |
| Mapuloteni mg / ml | ≥20 mg / ml |
| RNase | Palibe chomwe chapezeka |
| DNase | Palibe chomwe chapezeka |
| Nicase | Palibe chomwe chapezeka |
Mapulogalamu
genetic diagnostic zida;
RNA ndi DNA m'zigawo zida;
Kuchotsa zinthu zopanda mapuloteni kuchokera kuzinthu, kuwonongeka kwa zonyansa zamapuloteni, monga
DNA katemera ndi kukonzekera heparin;
Kukonzekera kwa chromosome DNA ndi pulsed electrophoresis;
Western blot;
Enzymatic glycosylated albumin reagents mu vitro diagnostics
Kutumiza ndi Kusunga
Manyamulidwe:Wozungulira
Zosungirako:Sungani pa -20 ℃(Nthawi Yaitali)/ 2-8℃(Kanthawi kochepa)
Tsiku loyesereranso lovomerezeka:zaka 2
