Uracil DNA Glycoylase (yopanda Glycerol)
Kufotokozera
Thermosensitive UDG (uracil-DNA glycosylase) imatha kuyambitsa hydrolysis ya uracil base ya DNA chain yokhala ndi uracil ndi N-glycosidic chomangira cha msana wa shuga-phosphate kuti amasule uracil waulere.Poyerekeza ndi ma enzyme wamba a UDG, ma enzymes a UDG a thermosensitive amapewa ntchito yotsalira ya ma enzymes wamba a UDG atasiya kugwira ntchito, zomwe zimatha kusokoneza zinthu zokulitsa zomwe zili ndi dU kutentha kwapakati.Izi zimagwira ntchito kutentha kwa chipinda ndipo sizimatentha komanso zimakhala zosavuta kuzimitsa.
Kapangidwe ka Chemical
Kufotokozera
| Enzyme | Glycosylase |
| Yogwirizana Buffer | Kusungirako Buffer |
| Kutentha Kwambiri | 50 ° C, 10 min |
| Tanthauzo la Chigawo | Chigawo chimodzi (U) chimatanthauzidwa ngati kuchuluka kwa enzyme yofunikira kuti ipangitse hydrolysis ya 1 μg dU-containing dsDNA mu mphindi 30 pa 25 ° C. |
Mapulogalamu
Chotsani kuipitsidwa kwa aerosol yokhala ndi PCR yokhala ndi dU.
Kuchotsa maziko a uracil ku DNA ya chingwe chimodzi kapena iwiri
Kutumiza ndi Kusunga
Mayendedwe:Mapaketi a ayezi
Zosungirako:Sungani pa -15 ℃ ~ -25 ℃
Moyo wa Shief:1 zaka
zokhudzana ndi mankhwala
Lembani uthenga wanu apa ndikutumiza kwa ife
