Gawo limodzi la RT-qPCR SYBR Green Kit

Universal Blue qPCR Master Mix (Dye Based) ndi yankho lakale la 2 × real-time quantitative quantitative PCR yodziwika ndi kukhudzika kwakukulu komanso kusiyanasiyana, ndi yabuluu mumtundu, ndipo imakhala ndi zotsatira za kutsata zitsanzo zowonjezera.Chigawo chapakati cha Taq DNA polymerase chimagwiritsa ntchito ma antibody otentha poyambira kuti aletse bwino kukulitsa kosatchulika chifukwa cha kuyambika koyambirira panthawi yokonzekera zitsanzo.Nthawi yomweyo, chilinganizocho chimawonjezera zinthu zomwe zimalimbikitsa kukulitsa mphamvu ya PCR ndikufananitsa kukulitsa kwa majini okhala ndi zinthu zosiyanasiyana za GC (30 ~ 70%), kotero kuti kuchuluka kwa PCR kumatha kupeza ubale wabwino wa mzere mu kuchuluka kwakukulu. dera.Izi zili ndi ROX Passive Reference Dye yapadera, yomwe imagwira ntchito pazida zonse za qPCR.Sikofunikira kusintha kuchuluka kwa ROX pazida zosiyanasiyana.Ndikofunikira kuwonjezera zoyambira ndi ma templates kuti mukonzekere kachitidwe kakukulitsa.