Gawo limodzi la RT-qPCR probe kit
Kufotokozera
One Step qRT-PCR Probe Kit idapangidwira qPCR yomwe imagwiritsa ntchito RNA mwachindunji (monga virus RNA) ngati template.Pogwiritsa ntchito ma gene specific primers (GSP), zolembera m'mbuyo ndi qPCR zitha kumalizidwa mu chubu chimodzi, kuchepetsa kwambiri njira zamapaipi komanso chiwopsezo cha kuipitsidwa.Itha kuyimitsidwa pa 55 ℃ popanda kukhudza mphamvu ndi chidwi cha qRT-PCR.Gawo limodzi la qRT-PCR Probe Kit limaperekedwa mu Master Mix.The 5 × One Step Mix ili ndi chosungira chokongoletsedwa bwino ndi dNTP/dUTP Mix, ndipo ndi yoyenera pazidziwitso zapamwamba kwambiri potengera ma probe olembedwa ndi fluorescence (monga TaqMan).
Mfundo Zoyambira za RT-qPCR
Kufotokozera
| Zinthu Zoyesa | Kufotokozera | Zotsatira |
| (SDS PAGE) Purity of Enzyme Stock(SDS PAGE) | ≥95% | Pitani |
| Endonuclease ntchito | Sizinazindikirike | Pitani |
| Ntchito ya Exodulease | Sizinazindikirike | Pitani |
| Ntchito ya Rnase | Sizinazindikirike | Pitani |
| DNA yotsalira ya E.coli | 1 makope/60 | Pitani |
| Functional Assay-System | 90%≤110% | Pitani |
Zigawo
| Zigawo | 100rx pa | 1,000rns | 5,000 rxns |
| RNase-free ddH2O | 2 * 1 ml | 20 ml pa | 100 ml |
| 5 * kusakaniza kwa sitepe imodzi | 600μl | 6*1ml pa | 30 ml pa |
| Kusakaniza kwa enzyme imodzi | 150μl | 2 * 750μl | 7.5ml pa |
| 50 * ROX reference Dye 1 | 60μl pa | 600μl | 3 * 1 ml |
| 50 * ROX reference Dye 2 | 60μl pa | 600μl | 3 * 1 ml |
a.One-Step Buffer ikuphatikiza dNTP Mix ndi Mg2+.
b.Kuphatikiza kwa enzyme kumakhala ndi zosintha
transcriptase, Hot Start Taq DNA polymerase (antibody modification) ndi RNase inhibitor.
c.Amagwiritsidwa ntchito kukonza zolakwika za sinal za fluorescene pakati pa zitsime zosiyanasiyana.
d.ROX: Muyenera kusankha ma calibration molingana ndi mtundu wa chida choyesera.
Mapulogalamu
Kuzindikira kwa QPCR
Kutumiza ndi Kusunga
Manyamulidwe:Mapaketi a ayezi
Zosungirako:Sungani pa -20 ℃.
Moyo wa Shief:18 miyezi
