Uracil DNA Glycosylase (UDG/UNG)
Kufotokozera
Heat-Labile UDG (uracil DNA glycosylase) imathandizira kutulutsidwa kwa uracil waulere ku DNA yokhala ndi uracil mwa hydrolyzing chomangira cha N-glycoside pakati pa maziko a uracil ndi mafupa a shuga-phosphate.Poyerekeza ndi ma enzymes wamba a UDG, kutentha-Labile UDG kumatha kupewa kuwonongeka kwa zinthu zokulitsa zomwe zili ndi dU chifukwa cha ntchito yotsalira ya UDG yosagwiritsidwa ntchito kutentha.Heat-Labile UDG (uracil DNA glycosylase) imagwira ntchito kutentha kwa chipinda ndipo ndi thermolabile komanso yosavuta kuyimitsa.
Kapangidwe ka Chemical
Kufotokozera
| Enzyme | Glycosylase |
| Yogwirizana Buffer | Kusungirako Buffer |
| Kutentha Kwambiri | 50 ° C, 10 min |
| Tanthauzo la Chigawo | Chigawo chimodzi (U) chimatanthauzidwa ngati kuchuluka kwa enzyme yofunikira kuti ipangitse hydrolysis ya 1 μg dU-containing dsDNA mu mphindi 30 pa 25 ° C. |
Mapulogalamu
1.Kuphunzira kukonza DNA ndi kuzindikira masinthidwe.
2.Kuwonjezera mphamvu ya cloning ya zinthu za PCR.
3.Kuwonjezera mphamvu ya mutagenesis yoyendetsedwa ndi malo.
Kutumiza ndi Kusunga
Mayendedwe:Mapaketi a ayezi
Zosungirako:Sungani pa -15 ℃ ~ -25 ℃
Moyo wa Shief:zaka 2
