Multiplex One Step RT-qPCR Probe Kit (UDG Plus)
Kufotokozera
Multiplex One Step RT-qPCR Kit (UDG Plus) ndi chida chodziwira mwachangu cha RT-qPCR choyenera PCR ya single-plex kapena multi-plex quantitative pogwiritsa ntchito RNA ngati template (monga kachilombo ka RNA).Amagwiritsidwa ntchito kukulitsa DNA yamitundu iwiri kuchokera ku ma tempuleti a RNA amtundu umodzi kuti alole kuchulukitsidwa kwanthawi yeniyeni kwa zolinga za RNA.Mu sitepe yolemba m'mbuyo reverse transcriptase imapanga ma molekyulu a DNA (cDNA) ogwirizana ndi template ya RNA.
Njira Yochitira
Zigawo
| Zigawo | 200rxn | 1,000rns | 10,000 rxns |
| RNase-free ddH2O | 3 * 1 ml | 15ml ku | 150 ml |
| 5 * kusakaniza kwa sitepe imodzi | 800μl | 4 *1ml pa | 40 ml pa |
| Kusakaniza kwa enzyme imodzi | 200μl | 1 ml | 10 ml pa |
| 50 * ROX reference Dye 1 | 80ml | 400μl | 4 *1ml pa |
| 50 * ROX reference Dye 2 | 80ml | 400μl | 4 *1ml pa |
a.One-Step Buffer ikuphatikiza dNTP Mix ndi Mg2+.
b.Enzyme Mix imakhala ndi reverse transcriptase, Hot Start Taq DNA polymerase (antibody modification) ndi RNase inhibitor.
c.Amagwiritsidwa ntchito kukonza zolakwika za sinal za fluorescene pakati pa zitsime zosiyanasiyana.
c.ROX: Muyenera kusankha ma calibration molingana ndi mtundu wa chida choyesera.
Mapulogalamu
Kuzindikira tizilombo toyambitsa matenda
diagnostics ndi kafukufuku wa chotupa
Kuzindikira matenda a nyama
Matenda oyambirira a matenda obadwa nawo
Kuzindikira tizilombo toyambitsa matenda mu chakudya
Kutumiza ndi Kusunga
Mayendedwe:Mapaketi a ayezi
Zosungirako:Sungani pa -30 ~ -15 ℃.
Moyo wa Shief:1 zaka
