BspQI Restriction Endonuclease
Kufotokozera
BspQI ikhoza kufotokozedwanso mu E. coli yomwe imatha kuzindikira malo enieni ndipo amapangidwa motsatira miyezo ya GMP.Amagwiritsidwa ntchito kwambiri popanga mapu a majini, cloning ndi madera ena.Enzyme iyi imadula mwachangu DNA kuti ipangitse bwino ma jini.
5'······GGTC(N)1······3'
3'······CCAGAG(N)4······5'
Kapangidwe ka Chemical
Tanthauzo la Chigawo
Chigawo chimodzi chimatanthauzidwa ngati kuchuluka kwa enzyme yofunikira kuti idye 1 µg ya λ DNA mu 1 ora pa 50 ° C mu chiwerengero chonse cha 50 µl.
Zogulitsa Zamankhwala
• Endonuclease yoletsa imakhala ndi ntchito yambiri ya enzymatic ndipo imamaliza chimbudzi mwachangu.
• Ntchito yotsika yopanda endonuclease yotsika kuti muwonetsetse kudula "scalpel" molondola.
• Palibe BSA dongosolo, kuchepetsa kutentha gwero kuipitsa.
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Mapuloteni Purity | ＞98% |
| Zotsalira za Protease | Palibe chomwe chapezeka |
| Kuzindikira kolumikizana ndi kudula | Kuchita bwino kwa digestion ＞95% |
| DNase | Palibe chomwe chapezeka |
| Nicakase | Palibe chomwe chapezeka |
Mayendedwe ndi kusunga
Mayendedwe:Kutumizidwa pansi pa 0 °C
Posungira:Sungani pa -20 ° C
Moyo woyesereranso wovomerezeka:2 chaka
