Multiplex PCR Taq DNA Polymerase

Multiplex PCR DNA Polymerase ndi m'badwo watsopano wa DNA polymerase yoyambira yotentha yotengera kusinthidwa kwa antibody ndikusinthidwa kuti igwirizane ndi ma template.Mtundu wa High Sensitivity wakonzedwa mosamalitsa kwa multiplex PCR yokhala ndi kuthekera kokulirapo komanso kuzindikira kwakukulu.Ndi dongosolo labwino kwambiri la buffer la multiplex PCR, limagwirizana ndi zinthu zambiri zamtundu wa GC komanso zoyambira za Tm.Kuyesa kochulukira kwa PCR kumatha kuchitidwa pa kutentha kwapadziko lonse kwa 60 ° C popanda kukhathamiritsa kwina.

Izi zimakhala ndi mphamvu zokulitsa kwambiri, ndipo nthawi imodzi zimatha kukulitsa zidutswa zandalama za 50 - 3,500 bp;ali ndi mphamvu zokulitsa, ndipo amatha kukulitsa mazana angapo kapena kupitilira apo;kulolerana bwino kwa zonyansa, kugonjetsedwa kwambiri ndi mitundu yosiyanasiyana ya zoletsa ndi zonyansa, komanso kumagwirizana ndi kukulitsa kwachindunji kwa magazi, makadi a magazi, ndi zina zotero. Ma reagents ali ndi kukhazikika kwabwino, kugwiritsidwa ntchito kwakukulu, ndipo amagwirizana ndi zochitika zosiyanasiyana zozindikiritsa.