Ultra Nuclease
Kufotokozera
UltraNuclease ndi endonuclaese yopangidwa ndi ma genetic yochokera ku Serratia marcescens, yomwe imatha kuwononga DNA kapena RNA, yokhala ndi mizere iwiri kapena yozungulira, yozungulira kapena yozungulira pansi pamikhalidwe yosiyanasiyana, imasokoneza ma nucleic acid kukhala 5'-monophosphate oligonucleotides yokhala ndi 3-5. kutalika kwa maziko.
Pambuyo genetic engineering kusinthidwa, mankhwala anali thovu, anasonyeza, ndi kuyeretsedwa mu Escherichia coli (E. coli), amene amachepetsa mamasukidwe akayendedwe a selo supernatant ndi selo lysate mu kafukufuku wa sayansi, komanso kusintha kuyeretsedwa dzuwa ndi ntchito kafukufuku wa mapuloteni.Itha kugwiritsidwanso ntchito mu mankhwala a jini, kuyeretsa ma virus, kupanga katemera, mapuloteni ndi polysaccharide pharmaceutical industry ngati zotsalira za nucleic acid kuchotsa reagent.
Kapangidwe ka Chemical
Tanthauzo la Chigawo
Kuchuluka kwa enzyme yomwe imagwiritsidwa ntchito posintha mayamwidwe a △A260 ndi 1.0 mkati mwa 30min pa 37 °C, pH 8.0, yofanana ndi 37μg ya umuna wa salimoni wopukutidwa mwa kudula kukhala oligonucleotides, idatanthauzidwa ngati gawo logwira ntchito.
Kugwiritsa Ntchito ndi Mlingo
• Chotsani exogenous nucleic acid ku mankhwala a katemera, kuchepetsa chiopsezo cha nucleic acid yotsalira kawopsedwe ndi kukonza chitetezo cha mankhwala.
• Chepetsani kukhuthala kwa madzi a chakudya chifukwa cha nucleic acid, kufupikitsa nthawi yokonza ndikuwonjezera zokolola.
• Chotsani nucleic acid yomwe idakulungidwa (virus, inclusion body, etc.), yomwe imathandizira kutulutsidwa ndi kuyeretsa kwa tinthu.
• Chithandizo cha nyukiliya chikhoza kupititsa patsogolo kuthetsa ndi kubwezeretsanso chitsanzo cha column chromatography, electrophoresis ndi blotting analysis.
• Mu chithandizo cha majini, nucleic acid imachotsedwa kuti ipeze mavairasi oyeretsedwa a adeno.
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Kufotokozera | Zomveka komanso Zopanda Mtundu |
| Zochita | ≥ 250 U / ul |
| Zochitika zenizeni | ≥1.1*106U/mg |
| Chiyero (SDS-PAGE) | ≥ 99.0% |
| Ma Protease | Palibe chomwe chapezeka |
| Bioburden | ＜10 cfu/100,000U |
| Endotoxines(LAL-test) | ＜ 0.25EU/1,000U |
Mayendedwe ndi kusunga
Mayendedwe:Kutumizidwa pansi pa 0 °C
Posungira:Sungani kutentha kwa -25-15 ° C
Moyo woyesereranso wovomerezeka:Zaka 2 (peŵani kuzizira)
