Superstart qPCR Premix kuphatikiza-UNG

Nambala ya mphaka: HCB5071E

Superstart qPCR Premix plus-UNG ndi reagent yapadera yomwe idapangidwira Real Time PCR mwatsatanetsatane komanso kachulukidwe kachulukidwe kake pogwiritsa ntchito kuzindikira kozikidwa pa probe, yopangidwira njira za lyophilization.Ili ndi puloteni yoyambira yotentha yotchedwa Hotstart Taq plus (DG), yomwe ili ndi ntchito yake ya Taq enzyme yosindikizidwa kutentha kwa firiji, ndikuletsa bwino kukulitsa kosakhazikika komwe kumachitika chifukwa cha kuyambika kosakhazikika kapena kupangika kwa dimer pansi pa kutentha kocheperako, motero kuwongolera. kukhazikika kwa kachitidwe ka amplification.Reagent iyi imagwiritsa ntchito nkhokwe yokhazikika ya qPCR ndi UNG/dUTP anti-contamination system kuti ifike mwachangu, kuwongolera bwino komanso kukhudzika kwa machitidwe a qPCR.Itha kupeza ma curve abwino okhazikika m'malo osiyanasiyana owerengera ndikuwerengera molondola, kuteteza bwino kukulitsa kwabodza komwe kumachitika chifukwa cha zotsalira za PCR kapena kuipitsidwa kwa aerosol.Reagent iyi imagwirizana ndi zida zambiri za PCR za fulorosenti zochokera kwa opanga monga Applied Biosystems, Eppendorf, Bio- Rad ndi Roche etc., ndipo amawonetsa kukhazikika kwabwino mu mawonekedwe a lyophilized.

2. Nambala ya kopi ya jini yomwe mukufuna yomwe ili mumitundu yosiyanasiyana ya ma templates imasiyanasiyana;ngati kuli kofunikira, dilution ya gradient imatha kuchitidwa kuti mudziwe kuchuluka kwa ma template owonjezera.

3. Dongosololi likhoza kukhala lyophilized;pamene makasitomala ntchito dongosolo popanda kuzizira-kuyanika zofunika, 4 × lyoprotectant akhoza kusankha anawonjezera; ngati pali amaundana zouma mankhwala chofunika, pa madzi reagents siteji mankhwala ntchito kutsimikizira, ayenera kuwonjezera 4 × lyoprotectant kuonetsetsa kugwirizana ndi lyophilized dongosolo zigawo zikuluzikulu. ndi zotsatira.

2. Onjezani template ya nucleic acid ku ufa wa lyophilized ndikuwonjezera madzi mpaka 25µL;

3. Sakanizani bwino ndi centrifugation ndi kuthamanga pa makina.

 Kuwongolera Ubwino:

1. Kuyesa kogwira ntchito: kukhudzika, kutsimikizika, kuchulukitsa kwa qPCR.

2. Palibe zochitika zamtundu wa nuclease, palibe kuipitsidwa kwa endo/exonuclease.

Zambiri Zaukadaulo:

1. Superstart qPCR Premix plus-UNG imagwiritsa ntchito puloteni yoyambira yotentha yomwe imathandizira kuyambitsa kutentha mwachangu mkati mwa mphindi 1 ~ 5;kudzera mukupanga kwapadera kwa bafa ndikoyenera ma multiplex fulorosenti kuchuluka kwa PCR reactions.

2. Ili ndi mawonekedwe apamwamba omwe amathandizira kwambiri kuzindikira kwa malire a fluorescence kuchuluka kwa PCR, kupanga ma curves amplification normalization, fluorescence mtengo kupeza kusintha koonekeratu pa otsika ndende zidindo, oyenera monga high sensitivity fluorescence kuchuluka PCR kuzindikira reagents.

3. Kwa zoyambira zokhala ndi kutentha kocheperako kapena kutalika kwa zidutswa za 200bp, njira ya masitepe atatu ndiyofunikira.

4. Kugwiritsa ntchito bwino kwa dUTP ndi kukhudzidwa kwa UNG enzyme kumasiyana pamitundu yosiyanasiyana ya chandamale, motero ngati kugwiritsa ntchito kachitidwe ka UNG kumabweretsa kuchepa kwa kuzindikira, kachitidwe kachitidwe kamayenera kusinthidwa ndikukonzedwa.Ngati thandizo laukadaulo likufunika chonde lemberani kampani yathu.

5. Gwiritsani ntchito madera odzipatulira ndi ma pipette musanayambe komanso mutatha kukulitsa, valani magolovesi pamene mukugwira ntchito ndikusintha nthawi zambiri;musatsegule chubu chotsatira mukamaliza PCR kuti muchepetse kuipitsidwa kwa zitsanzo ndi zinthu za PCR.