2 × HiF Taq kuphatikiza Master Mix

Nambala ya mphaka: HCR2014B

HIF Taq kuphatikiza Master Mix (Ndi Dye) ndi njira yokonzeka kugwiritsa ntchito 2× premixed yomwe ili ndi Plus HIF DNA Polymerase, dNTPs, ndi buffer yokhathamiritsa.Ma antibodies awiri a monoclonal pa kutentha kwa chipinda omwe amalepheretsa ntchito ya polymerase ndi 3′→5′exonuclease ntchito amawonjezedwa ku master mix mosavuta komanso mwachindunji Hot Start PCR.Chowonjezeracho chimawonjezedwa ku chisakanizo cha master kuti pulojekitiyi ikhale ndi mphamvu yokulirapo, kutalika kwa kukulitsa kumatha kufika 13 kb, enzyme imakhala ndi 5'→ 3' DNA polymerase ntchito ndi 3'→5' ntchito ya exonuclease, kukhulupirika kwake ndi nthawi 83 kuposa ya Taq DNA polymerase, yomwe ndi nthawi 9 kuposa ya DNA polymerase wamba.Ndikoyenera kukulitsa ma tempuleti ovuta, chokulitsacho chimakhala ndi mapeto osamveka.

2 × HIF Taq kuphatikiza Master Mix (Ndi Dye) ili ndi maubwino achangu komanso osavuta, okhudzika kwambiri, kukhazikika kwamphamvu, kukhazikika kwabwino, etc. sitepe protocol, kufewetsa masitepe oyesera ndikupulumutsa nthawi.Izi zili ndi utoto wowonetsa ma electrophoresis, ndipo zinthu za PCR zitha kugwiritsidwa ntchito mwachindunji pa electrophoresis.Kuphatikiza apo, mankhwalawa alinso ndi chinthu chodzitchinjiriza, kotero kuti kusakaniza kwa mbuyeyo kumatha kukhalabe ndi ntchito yokhazikika pambuyo pozizira mobwerezabwereza.

Zosungirako

Zogulitsa ziyenera kusungidwa pa -25 ~ -15 ℃ kwa chaka chimodzi.

Zofotokozera

Malangizo

1.PCR Reaction System

2.Kugwiritsiridwa ntchito kovomerezeka kwa ma tempulo osiyanasiyana

3.Amplification Protocol

1) Ndondomeko Yamagawo Awiri (chithunzi chovuta)

2) Ndondomeko Yamagawo Atatu (protocol yokhazikika)

3) Annealing Gradient Protocol (zovuta template)

Zomwe zili pansi pa ma protocol osiyanasiyana a amplification

Zolemba

Chonde valani PPE yofunikira, malaya a labu ndi magolovesi, kuti muwonetsetse thanzi lanu ndi chitetezo!