DNase I (Rnase Free) (5u/ul)

Nambala ya mphaka: HC4007A

Phukusi: 1000U/5000U/50000U

DNase I (Deoxyribonuclease I) ndi endodeoxyribonuclease yomwe imatha kugaya DNA ya zingwe ziwiri kapena ziwiri.

Mafotokozedwe Akatundu

Zambiri zamalonda

Nambala ya mphaka: HC4007A

DNase I (Deoxyribonuclease I) ndi endodeoxyribonuclease yomwe imatha kugaya DNA ya zingwe ziwiri kapena ziwiri.Imazindikira ndikudula zomangira za phosphodiester kuti ipange monodeoxynucleotides kapena oligodeoxynucleotides yokhala ndi nsonga imodzi kapena iwiri yokhala ndi magulu a phosphate pa 5'-terminal ndi hydroxyl pa 3'-terminal.Ntchito ya DNase I imadalira Ca²⁺ndipo imatha kuyendetsedwa ndi ayoni achitsulo a divalent monga Mn²⁺ndi zn²⁺.5 mm pa²⁺amateteza enzyme ku hydrolysis.Pamaso pa Mg²⁺, puloteniyo imatha kuzindikira mwachisawawa ndi kuswa malo alionse pa chingwe chilichonse cha DNA.Pamaso pa Mn²⁺, zingwe ziwiri za DNA zimatha kuzindikirika nthawi imodzi ndi kung'ambika pafupi ndi malo omwewo kuti apange zidutswa za DNA zathyathyathya kapena zidutswa za DNA zomata zokhala ndi ma nucleotides 1-2.

Zam'mbuyo: Chithunzi cha HC3905B

Ena: DNase I (Rnase Free) (2u/ul) HC4007B

Zigawo

Dzina	0.1KU	1 KU	5 KU	50 KU
DNase I, RNase-free	20μl pa	200μL	1ml pa	10 ml pa
10 × DNase I Buffer	1ml pa	1ml pa	5 × 1 mL	5 × 10 mL

Zosungirako

-25 ℃~-15 ℃ posungira;Transport pansi pa ayezi mapaketi.

Malangizo

1. Konzani njira yoyankhira mu chubu lopanda RNase molingana ndi magawo omwe ali pansipa:

Chigawo	Voliyumu
RNA	X µg
10 × DNase I Buffer	1 μl pa
DNase I, RNase-free(5U/μL)	1 U pa µg RNA①
ddH₂O	Mpaka 10 μL

Chidziwitso: ①Werengetsani kuchuluka kwa DNase I yomwe ikufunika kuwonjezeredwa kutengera kuchuluka kwa RNA.

2. 37 ℃ kwa mphindi 15;

3. Onjezani 0.5M EDTA ku ndende yomaliza ya 2.5mM ~ 5mM, ndi kutentha pa 65 ℃ kwa mphindi 10 kuti muyimitse zomwe zikuchitika.Chitsanzo chikhoza kugwiritsidwa ntchito mwachindunji pazotsatira zotsatila monga zolembera mobwererakuyesa.

Tanthauzo la Chigawo

Chigawo chimodzi chimatanthauzidwa ngati kuchuluka kwa enzyme yomwe ingawononge 1µg ya pBR322DNA mu mphindi 10 pa 37 ℃.

Kuwongolera Kwabwino

RNase:5U ya DNase I yokhala ndi 1.6µg MS2 RNA kwa maola 4 pa 37 ℃ sikutulutsa kuwonongeka mongakutsimikiziridwa ndi agarose gel electrophoresis.

Zolemba

1. Chonde konzani 0.5MEDTA nokha.

2. Gwiritsani ntchito 1U DNase I pa µg ya RNA.Komabe, ngati RNA ili yochepera 1µg, chonde gwiritsani ntchito 1U DNase I.

3. Chonde ikani enzyme pa ayezi panthawi yogwira ntchito.