Ribonuclease A (RNase A)

Ribonuclease A (RNase A) ndi polypeptide yokhala ndi chingwe chimodzi chokhala ndi 4 disulfide bond ndi molekyulu yolemera pafupifupi 13.7 kDa.RNase A ndi endoribonuclease yomwe imawononga kwambiri RNA yokhala ndi chingwe chimodzi pa C ndi U zotsalira.Mwachindunji, cleavage amazindikira phosphodiester mgwirizano wopangidwa ndi 5'-ribose wa nucleotide ndi phosphate gulu pa 3'-ribose wa moyandikana pyrimidine nucleotide, kotero kuti 2', 3' - Cyclic phosphates ndi hydrolyzed lolingana. 3'-nucleoside phosphates (mwachitsanzo, pG-pG-pC-pA-pG imadulidwa ndi RNase A kupanga pG-pG-pCp ndi A-PG).RNase A ndiyo yomwe imagwira ntchito kwambiri pakudula RNA yokhala ndi chingwe chimodzi ndipo imagwira ntchito mosiyanasiyana: pamchere wochepa (0 mpaka 100 mM NaCl), imatha kugwiritsidwa ntchito kung'amba RNA yokhala ndi chingwe chimodzi, RNA yokhala ndi zingwe ziwiri, ndi zingwe za RNA mu RNA-DNA hybrids.pomwe ili ndi mchere wambiri (≥0.3 M), RNase A imatha kudumpha RNA yokhala ndi chingwe chimodzi.