Recombinant Deoxyribonuclease I (DNase I, RNase-free)
Kufotokozera
Recombinant Deoxyribonuclease I, ndi endonuclease yomwe imatha kugaya DNA ya chingwe chimodzi kapena iwiri.Imatsitsa ma phosphodiester bond kuti apange mono- ndi oligodeoxynucleotides okhala ndi magulu a 5'-phosphate ndi magulu a 3'-OH.Mulingo woyenera kwambiri wa pH wa DNase I ndi 7-8.Ntchito ya DNase I imadalira Ca2 + ndipo ikhoza kuyendetsedwa ndi ma ion azitsulo a divalent monga Co2 +, Mn2 +, Zn2 +, ndi zina zotero. Pamaso pa Mg2 +, DNase ndingathe kudula DNA yamitundu iwiri mwachisawawa pamalo aliwonse;pamene pamaso pa Mn2 +, DNase Ine ndikhoza kudula DNA pawiri pa malo omwewo, kupanga malekezero osamveka kapena 1-2 nuclei Sticky mapeto ndi nucleotide overhangs.Enzyme iyi imachokera ku mitundu ina ya E. coli, ilibe RNase, ndipo ingagwiritsidwe ntchito pokonza zitsanzo zosiyanasiyana za RNA.
Kapangidwe ka mankhwala
Mapulogalamu
RNA m'zigawo: konzani RNA popanda DNA;
Pambuyo pa in vitro transcription ndi RNA polymerase, monga T7 RNA Polymerase (Cat#10618), imagwiritsidwa ntchito kuchotsa template ya DNA;
Konzani RNA yopanda DNA pamaso pa RT-PCR ndi RT-qPCR;
Amagwiritsidwa ntchito molumikizana ndi DNA Polymerase I (Mphaka#12903) polemba DNA mwa kumasulira kwa nick;
Amagwiritsidwa ntchito pofufuza mapazi kuti afufuze mgwirizano wa DNA-mapuloteni;
Pangani laibulale yachidutswa mwachisawawa;
Pakuzindikira kwa TUNEL kwa apoptosis, DNA yometedwa pang'ono idagwiritsidwa ntchito ngati chiwongolero chabwino.
Kutumiza ndi Kusunga
Mayendedwe:Mapaketi a ayezi
Zosungirako:Sungani mu -20 ℃
Shelf Life:zaka 2
Ndemanga:
1.Ma Enzymes ayenera kusungidwa mu bokosi la ayezi kapena pamadzi osambira akagwiritsidwa ntchito, ndipo ayenera kusungidwa pa -20 ° C mwamsanga mutatha kugwiritsa ntchito.
2.Pachitetezo chanu ndi thanzi lanu, chonde valani zida zodzitetezera (PPE), monga malaya a labotale ndi magolovesi otayika, mukamagwira ntchito ndi mankhwalawa.
