Cholesterol oxidase (COD/CHOD)
Kufotokozera
Cholesterol oxidase (CHOD) imathandizira gawo loyamba la cholesterol catabolism.Mabakiteriya ena osayambitsa matenda, monga Streptomyces amatha kugwiritsa ntchito cholesterol ngati gwero la kaboni.Tizilombo toyambitsa matenda, monga Rhodococcus equi, timafunika kuti CHOD ipatsire macrophage.CHOD simagwira ntchito ziwiri.Cholesterol poyamba imapangidwa ndi okosijeni kukhala cholest-5-en-3-imodzi mu sitepe yofunikira FAD.Cholest-5-en-3-one imasinthidwa kukhala cholest-4-en3-one.Zochita za CHOD zimatengera mawonekedwe a nembanemba omwe gawo lapansi limamangidwa.
CHOD imagwiritsidwa ntchito pozindikira cholesterol m'magazi.Ndi enzyme yachiwiri yomwe imagwiritsidwa ntchito kwambiri pazowunikira pambuyo pa glucose oxidase.CHOD imapezanso ntchito mu microanalysis ya steroids mu zitsanzo za zakudya komanso kusiyanitsa 3-ketosteroids kuchokera ku 3b-hydroxysteroids. Zomera za Transgenic zomwe zimasonyeza cholesterol oxidase zikufufuzidwa polimbana ndi thonje la thonje.Cholesterol oxidase imagwiritsidwanso ntchito ngati kafukufuku wa mamolekyulu kuti afotokozere zomwe zimachitika pama cell membrane.
Kapangidwe ka Chemical
Mfundo Yoti Muchite
Cholesterol + O2 →△4-Cholesten-3-imodzi + H2O2
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Kufotokozera | Yellow amorphous ufa, lyophilized |
| Zochita | ≥8U/mg |
| Chiyero(SDS-PAGE) | ≥90% |
| Kusungunuka (10mg ufa/ml) | Zomveka |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Mayendedwe ndi kusunga
Mayendedwe:Zotumizidwa pansi -15 ° C
Kusungirako :Sungani pa -25~-15°C(Nthawi Yaitali), 2-8°C(Kanthawi kochepa)
Kuyesedwanso kovomerezekaMoyo:1 chaka
