Glucose Dehydrogenase (GDH)
Kufotokozera
Glutamate Dehydrogenase (GDH) ndi puloteni ya mitochondrial yomwe imayambitsa kusinthika kwa okosijeni ya glutamate ku a-ketoglutarate ndipo imakhala ngati ulalo waukulu pakati pa anabolic ndi njira za catabolic.Mu zinyama, GDH imayang'aniridwa ndi allosteric regulation ndipo imakhala ndi ntchito zambiri m'chiwindi, impso, ubongo, ndi kapamba.Ntchito ya GDH mu seramu ingagwiritsidwe ntchito kusiyanitsa pakati pa matenda a chiwindi chifukwa cha kutupa kwa chiwindi, zomwe siziwonetsa ntchito yapamwamba ya GDH ya seramu, ndi matenda omwe amachititsa hepatocyte necrosis, zomwe zimapangitsa kuti seramu ya GDH ikhale yokwera.
Zochita za GDH zimatsimikiziridwa ndi kuyesa kwa enzyme komwe glutamate imadyedwa ndi GDH yopanga NADH, yomwe imachita ndi kafukufuku wotulutsa mtundu wa colorimetric (450 nm) molingana ndi zomwe GDH ilipo.Chigawo chimodzi cha GDH ndi kuchuluka kwa enzyme yomwe imapanga 1.0 mmol ya NADH pamphindi pa pH 7.6 pa 37 °C.
Kapangidwe ka Chemical
Reaction limagwirira
D-Glucose + wolandila → D-Glucono-1,5-lactone + wolandila wochepetsedwa
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Kufotokozera | White amorphous ufa, lyophilized |
| Zochita | ≥160U/mg |
| Chiyero(SDS-PAGE) | ≥90% |
| Kusungunuka (10 mg ufa/mL) | Zomveka |
| Kusokoneza ma enzyme | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Mayendedwe ndi kusunga
Mayendedwe: Mapaketi a ayezi
Kusungirako :Sungani pa -25~-15°C(Nthawi Yaitali), 2-8°C (nthawi yochepa)
Kuyesedwanso kovomerezekaMoyo: 18 miyezi
