Peroxidase (gwero la Horseradish) Mawu ofanana: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) imasiyanitsidwa ndi mizu ya horseradish (Amoracia rusticana) ndipo ndi ya gulu la ferroprotoporphyrin la peroxidases.HRP imasakanikirana mosavuta ndi hydrogen peroxide (H2O2).Zotsatira zake [HRP-H2O2] zovuta zimatha kutulutsa oxidize osiyanasiyana opereka haidrojeni:
Wopereka + H2O2 → Wopereka Oxidized + 2 H2O
HRP idzawonjezera magawo osiyanasiyana (onani Gulu 1):
• Chromogenic
• Chemiluminescent (monga luminol kapena isoluminol)
• Fluorogenic (monga tyramine, homovanillic acid, kapena 4-hydroxyphenyl acetic acid)
HRP ndi polypeptide imodzi yomwe imakhala ndi milatho inayi ya disulfide.HRP ndi glycoprotein yomwe ili ndi 18% yamafuta.Kapangidwe kazakudya kamakhala ndi galactose, arabinose, xylose, fucose, mannose, mannosamine, ndi galactosamine, kutengera isozyme yeniyeni.

HRP ndi chizindikiro chogwiritsidwa ntchito kwambiri cha ma immunoglobulins m'machitidwe osiyanasiyana a immunochemistry, kuphatikiza immunoblotting, immunohistochemistry, ndi ELISA.HRP imatha kulumikizidwa ku ma antibodies ndi njira zingapo zosiyanasiyana, kuphatikiza glutaraldehyde, okosijeni wanthawi zonse, kudzera mu zomangira za disulfide, komanso kudzera ma amino ndi thiol omwe amawongolera zolumikizira.HRP ndiye chizindikiro chofunidwa kwambiri cha ma antibodies, popeza ndi chocheperako komanso chokhazikika pa zilembo zitatu zodziwika bwino za ma enzyme (peroxidase, β-galactosidase, alkaline phosphatase) ndipo glycosylation yake imabweretsa kutsika kosagwirizana kwenikweni.Ndemanga ya glutaraldehyde ndi njira zolumikizirana zanthawi yayitali zasindikizidwa.

Peroxidase imagwiritsidwanso ntchito pozindikira glucose4 ndi peroxides mu yankho.Zolemba zingapo, mfundo za 6-24, 25-29 ndi zolemba 30-46 zatchulapo kugwiritsa ntchito P8375 muzofufuza zawo.