Uricase(UA-R) kuchokera ku Microorganism
Kufotokozera
Enzyme iyi ndiyothandiza pakuwunika kwachipatala kwa enzymatic determi nation ya uric acid.Uricase amatenga nawo gawo mu purine catabolism.Amathandizira kutembenuka kwa uric acid wosasungunuka kwambiri kukhala 5-hydroxyisourate.Kuchuluka kwa uric acid kumayambitsa kuwonongeka kwa chiwindi / impso kapena kumayambitsa gout.Mu mbewa, kusintha kwa jini komwe kumayambitsa uricase kumayambitsa kuwonjezereka kwadzidzidzi kwa uric acid.Makoswe, omwe alibe jini iyi, amawonetsa hyperuricemia, hyperuricosuria, ndi uric acid crystalline obstructive nephropathy.
Kapangidwe ka Chemical
Mfundo Yoti Muchite
Uric acid + O2+ 2H2O→ Allantoin + CO2+H2O2
Kufotokozera
Zinthu Zoyesa | Zofotokozera |
Kufotokozera | White amorphous ufa, lyophilized |
Zochita | ≥20U/mg |
Chiyero(SDS-PAGE) | ≥90% |
Kusungunuka (10mg ufa/ml) | Zomveka |
Kuphatikiza ma enzyme | |
NADH/NADPH oxidase | ≤0.01% |
Catalase | ≤0.03% |
Mayendedwe ndi kusunga
Mayendedwe:Kutumizidwa pansi pa -20 ° C
Kusungirako :Sungani pa -20°C(Nthawi Yaitali), 2-8°C(Kanthawi kochepa)
Kuyesedwanso kovomerezekaMoyo:2 chaka