Gawo la AMPPD-Chemiluminescent
Kufotokozera
AMPPD ili ndi magawo awiri ofunikira mu kapangidwe kake ka maselo.Imodzi ndi dioksijeni
mphete ya tetrad yomwe imalumikiza mphete ya benzene ndi adamantane, yomwe imatha kuthyoka ndikutulutsa mafotoni.Zina ndi kukhalapo kwa alkaline phosphatase, yomwe imayambitsa kuchotsedwa kwa magulu a phosphate ndikupanga kusakhazikika kwapakatikati.Wapakatikati ndiye adawola modzidzimutsa ndikutulutsa mafotoni, ndipo kuchuluka kwa mafotoni omwe adatulutsidwa kunali kolingana ndi kuchuluka kwa ALP mu yankho, lomwe linali loyenera pa chemiluminescence immunoassay system ndi ALP ngati enzyme yolembedwa.
Gwiritsani ntchito:
AMPPD ndi madzi a chemiluminescent omwe ali ndi chidwi kwambiri, kukhazikika komanso chiŵerengero cha ma signal-to-noise, omwe angagwiritsidwe ntchito pozindikiritsa chitetezo cha Alkaline Phosphatase (ALP) ngati chikhomo.
Kapangidwe ka Chemical
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Kufotokozera | Yellow wobiriwira bwino madzi |
| Kukhazikika | Mtengo wa Iuminescence wa 37 ℃ unasungidwa pamwamba pa 90% pambuyo pa masiku 7 akuyamwitsa pansi pa chitetezo cha kuwala. |
| pH mtengo | 9.5±0.05 |
| Mtengo wakumbuyo | 200-10000 |
| Luminecence mtengo mkulu ndende CV mtengo (poyerekeza ndi muyezo, mmene zinthu zinali 150 + 5μL, ndi ndende enzyme anali 0.0032U/mL). | ≤10% |
Mayendedwe ndi kusunga
Mayendedwe:Wozungulira
Kusungirako :Sungani kutentha kwa 2-8 ° C, kutetezedwa ku kuwala
Moyo woyesereranso wovomerezeka:2 chaka
