Ribonuclease III (RNase III)
Kufotokozera
Izi ndi ribonuclease III (RNase III) zomwe zimafotokozedwanso ndi E. coli.Exonuclease yeniyeniyi imadula RNA (dsRNA) yamitundu iwiri ndikupanga zidutswa za 12-35bp dsRNA zokhala ndi 5'-PO4 ndi 3'-OH, 3' overhangs.
Kapangidwe ka Chemical
Tanthauzo la Chigawo
Tanthauzo la gawo la zochitika: Gawo limodzi limatanthawuza kuchuluka kwa enzyme yofunikira kuti iwononge 1 μg ya
dsRNA kupita ku siRNA mu 50 μL reaction system pa 37 ° C kwa mphindi 20.
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Ntchito ya Exonuclease | Kutulutsidwa ＜ 0.1% ya ma radioactivity onse |
| Zochita Zosakhala Zapadera za Dnase | Osazindikirika |
| Protein Purity Assay (SDS-PAGE) | ≥ 95% |
| Ntchito ya RNase (Kuwonjezera Digestion) | ＞90% ya gawo lapansi la RNA limakhalabe |
Mayendedwe ndi kusunga
Mayendedwe:Owuma ayezi
Posungira:Sungani kutentha kwa -25-15 ° C
Moyo woyesereranso wovomerezeka:2 chaka
zokhudzana ndi mankhwala
Lembani uthenga wanu apa ndikutumiza kwa ife
