Poly A Carrier RNA
Nambala ya mphaka: HC4001A
Poly A, polyadenylate, ndi chisakanizo cha 100 ~ 10000 polyadenylates, yomwe imapangidwa ndi polynucleotide phosphorylase in vitro.Mu vivo, poly (a) amawonjezeredwa ku 3-terminal ya mRNA ndi enzyme kuti apititse patsogolo kukhazikika kwa mRNA.Pogwiritsa ntchito nucleic acid m'zigawo, kuwonjezera poly A ku lysate kapena njira yomangiriza kungapangitse zokolola za DNA ndi RNA.Njirapoly A kupititsa patsogolo zokolola za nucleic acid ndi motere:
1. Kukhudzana kwambiri ndi makondedwe apamwamba a zolemba.Zolemba zambiri za polypropylene zimakhala ndi magetsi osasunthika pamwamba, omwe amatsitsa ma nucleic acid.Carrier RNA imatha kukhutitsa iziadsorption zotsatira ndikuchepetsa kutayika kwa chandamale cha nucleic acid.
2. Imitsani ma nucleases: Pali ma nucleases osiyanasiyana m'masampuli achilengedwe ndichilengedwe.Poly A imatha kuletsa ma nucleases pochotsa kapena kusungakupititsa patsogolo zokolola ndi kukhazikika kwa chandamale cha nucleic acid.
3. Kuchuluka kwa mpweya: Pakuyeretsa kwa ma nucleic acid pakumwa mowa molumikizana ndi mpweya kapena kumangirira, poly A imatha kupitilira ndi chandamale cha nucleic acid kapena kupanga tinthu ta polima kutikusintha kuchira.
Zosungirako
-20 ~ 8 ℃, yosungirako youma, yosungirako nthawi yaitali iyenera kuikidwa pa -20 ℃
Kufotokozera
| Nambala ya CAS | 26763-19-9 |
| Maonekedwe | White lyophilized ufa |
| Chiyero | 99% |
| Kulemera kwa Maselo | 700-3500 KDa |
Njira yogwiritsira ntchito
Tengani mlingo woyenera wa lyophilized ufa, kuwonjezera DEPC mankhwala madzi kapena guanidine mchere njiraSungunulani mu 0.1-1ug/uL, ndiyeno perekani pang'ono ndikusunga pa -20 ° C.
Mapulogalamu
1.Virus DNA / RNA m'zigawo: kuwonjezera 1-5ug Wonyamula RNA ku lysate akhoza kusintha zokolola za RNA / DNA, kukhazikika kwa chandamale cha nucleic acid ndikupewa kuwonongeka kwa nucleic acid yoyeretsedwa panthawi yosungirako.
2. Mu micro DNA / RNA m'zigawo ndi njira ya membrane membrane (<1ug), kuwonjezera chonyamulira RNA ku 1-5ug ndi bwino kupititsa patsogolo zokolola za nucleic acid.
3. Mu mowa mediated nucleic asidi mpweya ndi ndende sitepe, Kuwonjezera 1-2ug chonyamulira RNA kumathandiza kusintha kuchira yochepa gawo RNA.
4. Mu kachulukidwe kafukufuku PCR anachita njira, kuwonjezera 10-100ng chonyamulira RNA kuti yankho yankho kumathandiza kusintha tilinazo ndi kuchepetsa C.Tmtengo.
