RT-LAMP Colormetric (mpira wa Lyophilized)

Mankhwalawa ali ndi zoteteza, RT-Enzymes Mix (Bst DNA polymerase ndi reverse transcriptase), zoteteza lyophilized ndi zigawo za utoto wa chromogenic.The mankhwala ndi lyophilized mpira mtundu, ntchito ndi zoyambira ndi zidindo.Chida ichi chimapereka chidziwitso chofulumira, chowonekera bwino cha kukulitsa, komwe kumapangitsa kuti kukhale koyipa kumasonyezedwa muzofiira komanso zabwino zimasonyezedwa ndi kusintha kwachikasu.

Chigawo

RT-LAMP Colormetric Master Mix (mikanda ya Lyophilized)

Mapulogalamu

Kwa DNA kapena RNA isothermal amplification.

Zosungirako

Kunyamulidwa ndikusungidwa pa 2 ~ 8 ℃.Zogulitsazo ndizovomerezeka kwa miyezi 12.

Ndondomeko

1.Chotsani nambala yofananira Lyophilized mikanda ufa molingana ndi kuchuluka kwa mayeso.

2.Konzani zosakaniza

Ndemanga:

1. 10 × Primer Mix Concentration: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;

2. Ma templates a Nucleic acid akulimbikitsidwa kuti asungunuke pogwiritsa ntchito madzi a DEPC.

3.Yalirani pa 65 ° C kwa 30-45mins, yomwe imatha kukulitsidwa moyenera molingana ndi kusintha kwa mtundu Nthawi yochitira.

4.Malinga ndi maso, chikasu chinali chabwino ndipo chofiira chinali choyipa.

Zolemba

1.Kutentha kwamachitidwe kumatha kukonzedwa pakati pa 62 ℃ ndi 68 ℃ malinga ndi momwe zilili.

2.The mmatumba reagents sayenera poyera mpweya kwa nthawi yaitali.

3.Maonekedwe ofiira ndi achikasu amadalira kusintha kwa pH kwa machitidwe, chonde musagwiritse ntchito njira yosungiramo ya Tris nucleic acid, yomwe ikulimbikitsidwa kugwiritsa ntchito ddH.₂O kusungidwa nucleic acid.

4.Kuyesera kudzachitika m'njira yovomerezeka, kuphatikizapo kukonzekera machitidwe, chithandizo chachitsanzo ndi kuwonjezera chitsanzo.

5.Ndibwino kuti tikonzekerere reaction system mu tebulo loyera kwambiri ndikuwonjezera ma templates mu fume hood ya zipinda zina kuti mupewe zabodza.