Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase ndi poyambira yotentha ya DNA polymerase yokhala ndi kutsekereza kawiri ndi ma antibodies awiri.Chida ichi sichimangotchinga ntchito ya 5′→3′ polymerase ya Taq DNA polymerase, komanso imatchinga ntchito ya 5′→3′exonuclease.Kutentha kwa masekondi 30 pa kutentha kwa pre-denaturation kumatha kuletsa antibody ndikumasula ntchito ya DNA polymerase ndi ntchito ya exonuclease.Mawonekedwe otsekereza kawiri sangangolepheretse kukulitsa kosafunikira komwe kumachitika chifukwa cha kusagwirizana kapena primer dimer, komanso kulepheretsa kutsika kwa siginecha ya fluorescence chifukwa cha kuwonongeka kwa probe, kuti ipangitse kuti in vitro detective reagent ikhale yokhazikika pamayendedwe kapena kugwiritsa ntchito chipinda. kutentha.
Zigawo
| Chigawo | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μl pa | 200 μL | 2 ml | 5ml pa |
Mkhalidwe Wosungira
Mankhwalawa amatumizidwa ndi ayezi wouma ndipo amatha kusungidwa pa -25 ° C ~ -15 ° C kwa zaka 2.
Zofotokozera
| Polymerase | Taq DNA Polymerase |
| Chiyero | ≥ 95% (SDS-PAGE) |
| Hot Start | Yomanga-Mu Hot Start |
| Kuthamanga Kwambiri | Standard |
| Ntchito ya Exonuclease | 5′→3′ |
Malangizo
Kukonzekera kwa Reaction
| Zigawo | Voliyumu (μL) | Kukhazikika Kwambiri |
| 2 × Bafaa | 25 | 1 × pa |
| Kusakaniza kwa Primer / Probeb | × | 0.1 μmol/L-0.5 μmol/L |
| Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| DNA Templatec | × | 0.1-100 ng |
| ddH2O | Mpaka 50 | - |
Ndemanga:
1) Malinga ndi ntchito yoyeserera, pamafunika kukonzekera chosungira chofananira.
2) Kuchuluka kwa DNA ndi kuchuluka kwa ma probes kapena zoyambira zimalimbikitsidwa.The mulingo woyenera kwambiri ndende akhoza kusinthidwa malinga ndi mikhalidwe yoyesera.
Thermal cycling protocol
| Khwerero | Kutentha(°C) | Nthawi | Zozungulira |
| Pre-denaturation | 95 ℃ | 5 mins | 1 |
| Denaturation | 95 ℃ | 15 sec | 45 |
| Zowonjezera / Zowonjezera | 60 ℃a | 30 secb |
Ndemanga:
1) Kutentha kwamachitidwe kumasinthidwa malinga ndi mtengo wa Tm wazomwe zidapangidwira.
2) Zida zosiyanasiyana za qPCR zimafunikira nthawi yotengera siginecha ya fluorescence, chonde ikani molingana ndi nthawi yaifupi kwambiri.
Zolemba
Chonde valani PPE yofunikira, malaya a labu ndi magolovesi, kuti mutsimikizire thanzi lanu ndi chitetezo!
