Sample Release Reagent
Sample Release Reagent ndi ya mamolekyu a POCT owunikira.Pamakina awiri okulitsa mwachindunji LAMP ndi PCR yokulitsa, palibe chifukwa chochotsa nucleic acid.The lysate yaiwisi chitsanzo akhoza mwachindunji amakulitsa, chandamale jini akhoza wapezeka molondola, chitsanzo kudziwika nthawi akhoza zina kufupikitsidwa, amene mwangwiro zikugwirizana ndi ntchito zofunika za maselo POCT.Ndi oyenera swabs mphuno, swabs pakhosi ndi zina zitsanzo.Zitsanzo zosinthidwa zitha kugwiritsidwa ntchito mwachindunji pa nthawi yeniyeni ya fluorescence kuchuluka kwa PCR kapena kuzindikira kwa LAMP, ndipo zotsatira zomwezo monga njira zachidule zochotsera zitha kutheka popanda zovuta zochotsa nucleic acid.
Zosungirako
Transport ndi sitolo kutentha firiji.
Kuwongolera khalidwe
Kuzindikira kogwira ntchito - kuchuluka kwa qPCR: Makina a 800μl Sample Release Reagent adakulitsidwa
yokhala ndi makope 1000 a Novel Pseudovirus, chitsanzo chimodzi cha swab cha m'mphuno, chomwe chimapangitsa kuti ma curve ofanana ndiMakhalidwe a ΔCt mkati mwa ± 0.5 Ct.
Njira Yoyeserares
1. Tengani 800 μl Sample Release Reagent ndikufalitsa njira ya lysis mu chubu cha 1.5 mL
2. Tengani swab ya m'mphuno kapena pakhosi ndi swab;Njira yotsatsira mphuno: tengani swab wosabala ndikuyiyika m'mphuno, pang'onopang'ono pita mpaka 1.5 cm kuya, tembenuzani pang'onopang'ono ka 4 pamphuno kwa masekondi oposa 15. , ndiye kubwereza ntchito yemweyo pa ena mphuno patsekeke ndi swab yemweyo.Pathroat swab chitsanzo ndondomeko: kutenga wosabala swab ndi modekha, mwamsanga misozi pharyngeal tonsils ndi kumbuyo pharyngeal khoma 3 zina.
3.Ikani swab nthawi yomweyo mu chubu cha zitsanzo.Mutu wa swab uyenera kuzunguliridwa ndi kusakaniza muzitsulo zosungirako kwa masekondi osachepera 30 kuti zitsimikizire kuti chitsanzocho chachotsedwa kwathunthu mu chubu lachitsanzo.
4. Makulitsidwe kutentha firiji (20 ~ 25 ℃) kwa 1min, kukonzekera lysis chotchinga chatha.
5. Onse 25μl dongosolo RT-PCR ndi RT-LAMP anali n'zogwirizana ndi 10μl kuchuluka kwa template kuwonjezera kuyesera kudziwika.
Zolemba
1. Chiwerengero chochepa cha chitsanzo cha lysate cholunjika chofanana ndi swab imodzi chikhoza kusinthidwa kukhala 400μl, chomwe chingasinthidwe malinga ndi zosowa zoyesa.
2. Kamodzi chitsanzo kukonzedwa ndi chitsanzo kumasulidwa reagent, tikulimbikitsidwa kuchita gawo lotsatira la mayeso mwamsanga, nthawi yodikira nthawi ndi makamaka zosakwana 1 ora.
3. pH ya chitsanzo cha lysate ndi acidic, ndipo njira yodziwira iyenera kukhala ndi buffer inayake.Ndizoyenera kudziwa zambiri za PCR, RT-PCR, ndi LAMP fluorescence yokhala ndi pH buffer, koma sizoyenera kuzindikira zamtundu wa LAMP popanda buffer.
