RT-LAMP Fluorescent (mpira wa Lyophilized)
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) imakhala ndi buffer reaction, Bst DNA polymerase and heat-resistant reverse transcriptase, lyophilized protectant, ndi utoto wa fulorosenti.Mankhwalawa ali ngati mikanda ya lyophilized, ndipo zoyambira zokha ndi ma templates ziyenera kuwonjezeredwa kuti zigwiritsidwe ntchito.
Chigawo cha Reagent
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Kugwiritsa ntchito
Kwa DNA kapena RNA isothermal amplification
Zosungirako
Kuyenda ndi kusunga pa 2 ~ 8°C.
Alumali moyo: 12 miyezi.
Ndondomeko
1. Chotsani nambala yofananira ya mikanda ya lyophilized molingana ndi kuchuluka kwa mayeso.
2. Kukonzekera dongosolo lochitira
| Chigawo | Mlingo |
| RT-LAMP Fluorescent Master Mix (Ufa wa Lyophilized) | 2 mikanda |
| Utoto wa fluorescent | x μl |
| 10 × Primer Mix a | 5 ml |
| Zithunzi za DNA/RNA b | n ml |
| Zonse | 50 μl pa |
Ndemanga:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.The nucleic acid template ikulimbikitsidwa kuti isungunuke mumadzi a DEPC.
Malingaliro a kampani Hyasen Biotechnology Co., Ltd
3) Pulogalamu yochitira
| Kutentha | Nthawi | Zozungulira |
| 65 ℃ | 60s a | 30 |
Ndemanga:
1. Sungani chizindikiro cha fulorosenti
Zolemba
1. Kutentha komweko kumatha kukonzedwa pakati pa 62 ° C ndi 68 ° C malinga ndi choyambiriramikhalidwe;
2. Kuyesera kuyenera kuyimitsa ntchitoyo, kuphatikizapo kukonzekera kachitidwe kachitidwe,chitsanzo processing ndi dosing;
3. Pofuna kupewa kuipitsidwa, tikulimbikitsidwa kukonzekera kachitidwe kachitidwe patebulo loyera kwambirindi kuwonjezera ma templates ku hood ya fume mu zipinda zina kuti mupewe kusokonezedwa kwabodza.
