RT-LAMP Fluorescent Master Mix (Lyophilized Beads)
Mafotokozedwe Akatundu
LAMP pakali pano ikugwiritsidwa ntchito kwambiri ukadaulo pantchito ya isothermal amplification.Imagwiritsa ntchito zoyambira 4-6 zomwe zimatha kuzindikira madera 6 pamtundu womwe mukufuna, ndipo zimadalira ntchito yolimba ya Bst DNA polymerase.Pali njira zambiri zowunikira LAMP, kuphatikizapo njira ya utoto, pH colorimetric njira, turbidity njira, HNB, calcein, etc. RT-LAMP ndi mtundu umodzi wa LAMP reaction ndi RNA monga template.RT-LAMP Fluorescent Master Mix (Lyophilized Powder) ili mu mawonekedwe a Lyophilized Powder, ndipo imangofunika kuwonjezera zoyambira ndi ma templates poigwiritsa ntchito.
Kufotokozera
| Zinthu zoyesa | Zofotokozera |
| Endonulease | Palibe zosankhidwa |
| Ntchito ya RNase | Palibe chomwe chapezeka |
| DNase ntchito | Palibe chomwe chapezeka |
| Nickase ntchito | Palibe chomwe chapezeka |
| E. koli.gDNA | ≤10kopi / 500U |
Zigawo
Izi zili ndi Reaction Buffer, RT-Enzymes Mix ya Bst DNA Polymerase ndi Thermostable Reverse Transcriptase, Lyoprotectant ndi Fluorescent Dye Components.
Amplication
Isothermal amplification ya DNA ndi RNA.
Kutumiza Ndi Kusunga
Mayendedwe:Wozungulira
Zosungirako:Sungani pa -20 ℃
Tsiku loyesereranso lovomerezeka:18 Miyezi
