Virus DNA/RNA Extraction Kit
The zida (HC1009B) mwamsanga wachotsa mkulu-kuyeretsedwa tizilombo nucleic zidulo (DNA/RNA) zosiyanasiyana madzi zitsanzo monga magazi, seramu, plasma, ndi swab kutsuka madzi, kuwapangitsa mkulu-throughput processing wa zitsanzo kufanana.Chidacho chimagwiritsa ntchito mikanda yamaginito ya superparamagnetic silicon-based magnetic.Mu dongosolo lapadera la buffer, nucleic acids m'malo mwa mapuloteni ndi zonyansa zina zimatulutsidwa ndi ma hydrogen bond ndi electrostatic binding.Maginito mikanda amene adsorbed nucleic acid amatsukidwa kuchotsa mapuloteni otsala ndi mchere.Mukamagwiritsa ntchito mchere wocheperako, ma nucleic acid amatulutsidwa kuchokera ku mikanda ya maginito, kuti akwaniritse cholinga cholekanitsa mwachangu komanso kuyeretsa ma nucleic acid.Njira yonse yogwirira ntchito ndi yosavuta, yachangu, yotetezeka komanso yothandiza, ndipo ma nucleic acid omwe amapezeka amatha kugwiritsidwa ntchito mwachindunji pazoyeserera zakumunsi monga kulembera kumbuyo, PCR, qPCR, RT-PCR, RT-qPCR, kutsata mibadwo yotsatira, kusanthula kwa biochip, ndi zina.
Zosungirako
Sungani pa 15 ~ 25 ℃, ndikuyendetsa kutentha.
Mapulogalamu
Magazi, seramu, plasma, swab eluent, minofu homogenate ndi zina zambiri.
Njira Yoyesera
1. Chitsanzo kukonza
1.1 Pakuti mavairasi mu zitsanzo zamadzimadzi monga magazi, seramu, ndi plasma: 300μL wa supernatant ntchito m'zigawo.
2.2 Pazitsanzo za swab: Ikani zitsanzo za swab mu machubu achitsanzo omwe ali ndi njira yosungira, vortex kwa mphindi imodzi, ndikutenga 300μL supernatant kuti muzule.
1.3 Kwa ma virus omwe ali mu ma homogenates a minofu, mayankho a minyewa, ndi zitsanzo zachilengedwe: Imani zitsanzo kwa mphindi 5 -10, ndikutenga 300μL ya supernatant kuti muchotse.
2. Kukonzekera kwa kukonzekerareagent yokhazikika
Chotsani ma reagents omwe adapakidwa kale mu zida, tembenuzani ndikusakaniza kangapo kuti muyimitsenso mikanda ya maginito.Gwirani mbale pang'onopang'ono kuti ma reagents ndi mikanda ya maginito zimire pansi pa chitsime.Chonde tsimikizirani komwe mbaleyo ikulowera ndikung'amba mosamala zojambulazo zosindikizira za aluminiyamu.
Δ Pewani kugwedezeka pamene mukung'amba filimu yosindikiza kuti madzi asatayike.
3. Ntchito ya automchida cha atic
3.1 Onjezani 300μL ya zitsanzo ku zitsime mu Zigawo 1 kapena 7 za mbale zakuya za 96 (tcherani khutu ku malo ogwira ntchito bwino).Voliyumu yolowera yachitsanzo imagwirizana ndi 100-400 μL.
3.2 Ikani mbale yakuya ya 96-chitsime mu nucleic acids extractor.Valani manja a maginito, ndikuonetsetsa kuti akuphimba ndodo za maginito.
3.3 Khazikitsani pulogalamu motere kwa basi m'zigawo:
3.4 Pambuyo pochotsa, sinthani zomwe zili mu Columns 6 kapena 12 za 96 deep well plate (tcherani khutu ku malo ogwira ntchito bwino) kupita ku chubu choyera cha Nuclease-free centrifuge.Ngati simugwiritsa ntchito nthawi yomweyo, chonde sungani zinthuzo pa -20 ℃.
Zolemba
Kugwiritsa ntchito kafukufuku kokha.Osagwiritsidwa ntchito pozindikira matenda.
1. Zomwe zatulutsidwa ndi DNA/RNA.Chisamaliro chapadera chiyenera kuperekedwa pofuna kupewa kuwonongeka kwa RNA ndi RNase panthawi ya opaleshoni.Ziwiya ndi zitsanzo zomwe zimagwiritsidwa ntchito ziyenera kuperekedwa.Machubu onse ndi nsonga za pipette ziyenera kukhala zosawilitsidwa ndi DNase/RNase-free.Oyendetsa ayenera kuvala magolovesi opanda ufa ndi masks.
2. Chonde werengani buku la malangizo mosamala musanagwiritse ntchito, ndipo gwirani ntchito motsatira buku la malangizo.Kukonzekera kwachitsanzo kuyenera kuchitika mu benchi yoyera kwambiri kapena kabati yachitetezo chachilengedwe.
3. The automatic nucleic acid m'zigawo dongosolo ayenera disinfected ndi UV kwa 30 min isanayambe ndi pambuyo ntchito.
4. Pakhoza kukhala zizindikiro za mikanda ya maginito yotsalira mutatha kutulutsa, choncho pewani kulakalaka mikanda ya maginito.Ngati mikanda ya maginito ikufunika, imatha kuchotsedwa ndi maginito.
5. Ngati palibe malangizo apadera a magulu osiyanasiyana a reagents, chonde musawasakanize, ndipo onetsetsani kuti zidazo zimagwiritsidwa ntchito mkati mwa nthawi yovomerezeka.
6. Moyenera kutaya zitsanzo zonse ndi reagent, bwinobwino misozi pansi ndi mankhwala pamalo onse ntchito ndi 75% Mowa.
