Phosphatase Alkaline (ALP)
Kufotokozera
Alkaline Phosphatase amachokera ku mtundu wina wa E. coli womwe umanyamula jini ya TAB5.Enzyme imayambitsa dephosphorylation ya 5' ndi 3' kumapeto kwa DNA ndi RNA phosphomonoesters.Komanso, imatulutsa ribose, komanso deoxyribonucleoside triphosphates (NTPs ndi dNTPs).TAB5 Alkaline Phosphatase imagwira ntchito pa 5' yotuluka, 5' yotsalira komanso yosasinthika.Phosphatase imatha kugwiritsidwa ntchito pazinthu zambiri zama cell biology, monga kupanga cloning kapena probe end label kuchotsa ma phosphorylated a DNA kapena RNA.Pakuyesa kwa cloning, dephosphorylation imalepheretsa DNA ya plasmid yokhazikika kuti isadzipangire.Itha kutsitsanso ma dNTP osaphatikizidwa mu machitidwe a PCR kuti akonze template yotsatizana ndi DNA.Enzymeyi imakhala yosasunthika komanso yosasinthika chifukwa cha kutentha kwa 70 ° C kwa mphindi 5, motero kumapangitsa kuti phosphatase ichotsedwe musanayambe kulumikiza kapena kuletsa kulemba zilembo kukhala zosafunikira.
Kugwiritsa ntchito
1.Alkaline phosphatase yophatikizidwa ndi mapuloteni (antibodies, streptavidin etc,) amatha kuzindikira mwachindunji mamolekyu omwe amawunikira, ndipo angagwiritsidwe ntchito mu ELISA, WB ndi kuzindikira kwa histochemical;
2.Alkaline phosphatase angagwiritsidwe ntchito dephosphorize ndi 5 '-terminal ya DNA kapena RNA kuteteza kudzidalira;
3.Dephosphorylated DNA kapena RNA yomwe ili pamwambapa imatha kulembedwa ndi ma phosphates olembedwa ndi wailesi (kudzera T4 poly-nucleotide kinase)
Kapangidwe ka Chemical
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Ntchito ya enzyme | 5U/μL |
| Endonuclease ntchito | Sizinazindikirike |
| Ntchito ya Exonuclease | Sizinazindikirike |
| Nicking Activity | Sizinazindikirike |
| Ntchito ya RNase | Sizinazindikirike |
| E.coli DNA | ≤1kope/5U |
| Endotoxin | LAL-Mayeso, ≤10EU/mg |
| Chiyero | ≥95% |
Mayendedwe ndi kusunga
Mayendedwe:AmbIent
Kusungirako :Sungani pa 2-8 ° C
Kuyesedwanso kovomerezekaMoyo:2 chaka
