Khwerero Chimodzi qRT-PCR Probe Kit-Master Mix, Kuwunika kwa mamolekyulu a Chithunzi Chowonetsedwa

Gawo limodzi la RT-qPCR probe kit

Phukusi: 100rxns, 1000rxns, 5000rxns

Mafotokozedwe Akatundu

Kufotokozera

U+ One Step RT-qPCR Probe Kit (Glycerol-free) ndi glycerol-free sitepe RT-qPCR reagent yogwiritsa ntchito RNA monga template (monga RNA virus), yomwe ili yoyenera pakupanga ndi kupanga mankhwala a lyophilized.Izi zimaphatikiza magwiridwe antchito apamwamba a gawo limodzi lodzipatulira la Reverse Transcriptase ndi Champagne Taq DNA Polymerase yotentha, yokhala ndi zida zowumitsa zowumitsa bwino zamakemikolo, zomwe zimakhala ndi mphamvu zokulitsa bwino, moyenera komanso mwapadera, ndipo zimagwirizana ndi kuyanika kosiyanasiyana kozizira. Kuonjezera apo, dUTP / UDG anti-pollution system imalowetsedwa mu reagent, yomwe imatha kugwira ntchito kutentha kwa chipinda, kuthetsa zotsatira za kuipitsidwa kwa mankhwala okulitsa pa qPCR, ndikuwonetsetsa kuti zotsatira zake ndi zolondola.

Mfundo Zoyambira za RT-qPCR

Kufotokozera

Zinthu Zoyesa	Kufotokozera	Zotsatira
(SDS PAGE) Purity of Enzyme Stock(SDS PAGE)	≥95%	Pitani
Endonuclease ntchito	Sizinazindikirike	Pitani
Ntchito ya Exodulease	Sizinazindikirike	Pitani
Ntchito ya Rnase	Sizinazindikirike	Pitani
DNA yotsalira ya E.coli	1 makope/60	Pitani
Functional Assay-System	90%≤110%	Pitani

Zigawo

Zigawo	100rxn pa	1,000rns	5,000 rxns
RNase-free ddH2O	2 * 1 ml	20 ml pa	100 ml
5 * kusakaniza kwa sitepe imodzi	600μl	6*1ml pa	30 ml pa
Kusakaniza kwa enzyme imodzi	150μl	2 * 750μl	7.5ml pa
50 * ROX reference Dye 1	60μl pa	600μl	3 * 1 ml
50 * ROX reference Dye 2	60μl pa	600μl	3 * 1 ml