M-MLV Reverse Transcriptase (Glycerol yaulere)
Ndi lyophilizable Reverse Transcriptase.Itha kugwiritsidwa ntchito paukadaulo wa lyophilization wakumunsi ndikusunga magwiridwe antchito komanso kukhazikika.Izi zilibe zowonjezera, chonde onjezerani zanu ngati mukufunikira.
Zigawo
| Chigawo | HC2005 A-01 (10,000U) | HC2005 A-02 (40,000U) |
| Reverse Transcriptase (Glycerol Free) (200U/μL) | 50 μl pa | 200 μL |
| 5 × Kuwombera | 200 μL | 800 μl |
Ntchito:
Imagwira ntchito pamachitidwe amodzi a RT-qPCR.
Mkhalidwe Wosungira
Sungani pa -30 ~ -15 ° C ndi zoyendera pa ≤0 ° C.
Tanthauzo la Chigawo
Chigawo chimodzi (U) chimatanthauzidwa ngati kuchuluka kwa enzyme yomwe imaphatikizapo 1 nmol ya dTTP muzinthu zosasungunuka za asidi mu 10mins pa 37 ° C, ndi Poly(rA)·Oligo (dT) monga template / primer.
Zolemba
Kugwiritsa ntchito kafukufuku kokha.Osagwiritsidwa ntchito pozindikira matenda.
1.Chonde sungani malo oyesera kukhala oyera;Valani magolovesi otayika ndi masks;Gwiritsani ntchito zopangira zopanda RNase monga machubu apakati ndi malangizo a pipette.
2.Sungani RNA pa ayezi kuti mupewe kuwonongeka.
3.Ma tempulo apamwamba kwambiri a RNA amalimbikitsidwa kuti akwaniritse zolembedwa bwino kwambiri.
