Hexokinase (HK)
Kufotokozera
Gwiritsani ntchito Hexokinase kuti mudziwe za D-glucose, D-fructose, ndi D-sorbitol muzakudya kapena zitsanzo za kafukufuku wachilengedwe.Enzyme imagwiritsidwanso ntchito poyesa ma saccharides ena omwe amasinthidwa kukhala shuga kapena fructose, motero ndiwothandiza pakuyesa ma glycosides ambiri.
Ngati Hexokinase agwiritsidwa ntchito limodzi ndi glucose-6-mankwala dehydrogenase (G6P-DH)* (mayeso a glucose6-phosphate opangidwa ndi Hexokinase), zitsanzo siziyenera kukhala zamtundu wapamwamba wa mankwala chifukwa G6P-DH imaletsedwa mopikisana ndi mankwala.
Kapangidwe ka Chemical
Mfundo Yoti Muchite
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Kufotokozera | Zoyera mpaka zachikasu pang'ono amorphous ufa, lyophilized |
| Zochita | ≥30U/mg |
| Chiyero(SDS-PAGE) | ≥90% |
| Kusungunuka (10mg ufa/ml) | Zomveka |
| Ma Protease | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Mayendedwe ndi kusunga
Mayendedwe: Achozungulira
Kusungirako :Sungani pa -20°C(Nthawi yayitali), 2-8°C (nthawi yochepa)
Kuyesedwanso kovomerezekaMoyo:1 chaka
