Glycohemoglobin A1c (HbA1c) Test Kit
Ubwino wake
● Zolondola kwambiri
● Mphamvu yolimbana ndi kusokoneza
● Kukhazikika bwino
Kapangidwe ka Chemical
Mapulogalamu
Kuyesa kwa in vitro kuti mudziwe kuchuluka kwa kuchuluka kwa HbA1c m'magazi athunthu amunthu pamakina a photometric.HbA1c imapangidwa ndi hemoglobin (Hb) yomwe imapangitsa kuti glycation ichitike pang'onopang'ono komanso mosalekeza pansi pa kuchuluka kwa shuga m'magazi.Glucose amasintha hemoglobin makamaka mu n-terminal valine zotsalira kuti apange glycated hemoglobin.M'mikhalidwe yabwinobwino yokhudzana ndi thupi, kupanga zinthu zopanda enzymatic glycosylation reaction kumagwirizana bwino ndi kuchuluka kwa ma reactants.Popeza kuchuluka kwa hemoglobini kumakhalabe kokhazikika, milingo ya glycosylation imadalira kwambiri kuchuluka kwa shuga ndipo imagwirizananso ndi kutalika kwa hemoglobin ndi kuwonetsa kwa glucose.Chifukwa chake, HbA1c ndi chisonyezo chabwino cha kuchuluka kwa shuga m'magazi a odwala kwa miyezi 2-3 yapitayi.
Mfundo yofunika
Pansi pa protease, n-terminal ya β chain mu HbA1c imadulidwa ndipo dipeptide za glycosylated zimatulutsidwa.Poyambira koyamba, ndende ya Hb imatha kupezeka poyesa kuyamwa kwa 480 nm.Pochita chachiwiri, fructosyl peptide oxidase (FPOX) imagwira ntchito pa glycosylated dipeptides kuti ipange hydrogen peroxide yomwe imatha kuchitapo kanthu ndi chromogenic agents kuti ipange kuyamwa pa 660nm pamaso pa peroxidase, ndiye kuti kuchuluka kwa HbA1c kumatha kupezeka poyesa kuyamwa kwa 660nm pa.Malinga ndi kuchuluka kwa HbA1c komanso kuchuluka kwa Hb, kuchuluka kwa HbA1c (HbA1c%) kumatha kuwerengedwa.
Zotheka
Hitachi 7180/7170/7060/7600 automatic biochemical analyser, Abbot 16000, OLYMPUS AU640automatic biochemical analyzer
Ma reagents
| Zigawo | Malingaliro |
| Regent 1(R1) | |
| Ubwino wa buffer | 100 mmol / L |
| Chithunzi cha PRK | 500KU/L |
| DA-67 | 10 mmol / L |
| Reagents 2 (R2) | |
| Ubwino wa buffer | 100 mmol / L |
| Fructosyl peptide oxidase | 50 KU/L |
| Reagent 3(R3) | |
| Ubwino wa buffer | 100 mmol / L |
Mayendedwe ndi kusunga
Mayendedwe:Wozungulira
Kusunga ndi Kukhazikika:
Kufikira tsiku lotha ntchito lomwe lasonyezedwa pa cholembera, chosungidwa osatsegulidwa pa 2-8 ℃ ndikutetezedwa ku kuwala.Akatsegulidwa, ma reagents amakhala okhazikika kwa masiku 28 akasungidwa mufiriji pa analyzer kapena firiji.
Kuwonongeka kwa ma reagents kuyenera kupewedwa.Osaundana ma reagents.
Akasungunuka, calibrator imakhala yokhazikika kwa masiku 15 pa 2-8 ℃, kuwongolera kumakhala kokhazikika kwa masiku 7 pa 2-8 ℃, osazizira.
Shelf Life:1 chaka
