D-Lactate dehydrogenase (LDH)
Kufotokozera
Enzyme imagwiritsidwa ntchito pochotsa pyruvate pozindikira zomwe zimagwira ntchito ndi NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Kugwiritsa ntchito
1.Alkaline phosphatase yophatikizidwa ndi mapuloteni (antibodies, streptavidin etc,) amatha kuzindikira mwachindunji mamolekyu omwe amawunikira, ndipo angagwiritsidwe ntchito mu ELISA, WB ndi kuzindikira kwa histochemical;
2.Alkaline phosphatase angagwiritsidwe ntchito dephosphorize ndi 5 '-terminal ya DNA kapena RNA kuteteza kudzidalira;
3.Dephosphorylated DNA kapena RNA yomwe ili pamwambapa imatha kulembedwa ndi ma phosphates olembedwa ndi wailesi (kudzera T4 poly-nucleotide kinase)
Kapangidwe ka Chemical
Reaction limagwirira
D-Lactate + NAD+→ Pyruvate +NADH + H+
Kufotokozera
| Zinthu Zoyesa | Zofotokozera |
| Kufotokozera | White amorphous ufa, lyophilized |
| Zochita | ≥200U/mg |
| Chiyero(SDS-PAGE) | ≥90% |
| Kusungunuka (10mg ufa/mL) | Zomveka |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Mayendedwe ndi kusunga
Mayendedwe:AmbIent
Kusungirako :Sungani pa -20°C(Nthawi yayitali), 2-8°C (nthawi yochepa)
Kuyesedwanso kovomerezekaMoyo:2 chaka
