CHO HCP ELISA Kit

Nambala ya mphaka: HCP0032A

phukusi: 96T

Njira imodzi ya immunosorbent ELISA imagwiritsidwa ntchito poyesa izi.Zitsanzo zomwe zili ndi CHOK1 HCP nthawi imodzi zimachita ndi anti-CHOK1 antibody ya mbuzi yolembedwa ndi HRP ndi anti-CHOK1 antibody yomwe idakutidwa pa mbale ya ELISA.

Tumizani Kufunsira

Mafotokozedwe Akatundu

Tsiku la malonda

Njira imodzi ya immunosorbent ELISA imagwiritsidwa ntchito poyesa izi.Zitsanzo zomwe zili ndi CHOK1 HCP nthawi imodzi zimachita ndi anti-CHOK1 antibody yolembedwa ndi HRP ndi anti-CHOK1 yomwe idakutidwa pa mbale ya ELISA, pomaliza kupanga masangweji ophatikizika a antibody-phase-HCP-olembedwa ndi anti-HCP.Antigen-antibody osamangidwa amatha kuchotsedwa potsuka mbale ya ELISA.Gawo laling'ono la TMB limawonjezedwa pachitsime kuti muyankhe mokwanira.Kukula kwamtundu kumayimitsidwa pambuyo powonjezera njira yoyimitsa, ndipo OD kapena kuyamwa kwa mayankho pa 450/650nm kumawerengedwa ndi owerenga microplate.Mtengo wa OD kapena kuyamwa kumayenderana ndi zomwe zili mu HCP mu yankho.Kuchokera apa, ndende ya HCP mu yankho imatha kuwerengedwa molingana ndi mayendedwe okhazikika.

Zam'mbuyo: Mouse Genotyping Kit HCR2021A

Ena: RNA (dsRNA) yazingwe ziwiri ELISA KIT HCP0033A

Kugwiritsa ntchito

Chidachi chimagwiritsidwa ntchito kuti azindikire kuchuluka kwa zotsalira za CHOK1 zotsalira zama protein m'masampuli.

Cotsutsa

S/N	Chigawo	Kukhazikika	Zosungirako
1	CHOK1 HCP Standard	0.5mg/mL	≤–20 ℃
2	Anti-CHO HCP-HRP	0.5mg/mL	≤–20 ℃, tetezani ku kuwala
3	TMB	NA	2-8 ℃, kuteteza ku kuwala
4	20 × PBST 0.05%	NA	2-8 ℃
5	Imani njira	NA	RT
6	Microplate sealers	NA	RT
7	BSA	NA	2-8 ℃
8	Mkulu adsorption chisanadze ❖ kuyanika mbale	NA	2-8 ℃

Zida Zofunika

Consumables / Zida	Kupanga	Catalog
Wowerenga Microplate	Zida Zamagetsi	Spectra Max M5, M5e, kapena zofanana
Thermomixer	Eppendorf	Eppendorf/5355, kapena zofanana
Chosakaniza cha Vortex	IKA	MS3 Digital, kapena zofanana

Kusungirako ndi kukhazikika

1.Kuyenda pa -25 ~ 15 ° C.

2.Zosungirako zili monga momwe tawonetsera mu Gulu 1;zigawo 1-2 zimasungidwa ≤-20 ° C, 5-6 zimasungidwa RT, 3,4, 7, 8 amasungidwa pa 2-8 ℃;nthawi yovomerezeka ndi miyezi 12.

Mankhwala magawo

1.Kumverera: 1ng/mL

2.Kuzindikira osiyanasiyana: 3-100ng/mL

3.Kulondola: CV yoyeserera ≤ 10%, inter-assay CV≤ 15%

4.Kufalikira kwa HCP:> 80%

5.Kudziwikiratu: Chida ichi ndi chapadziko lonse lapansi chifukwa chimayenderana ndi CHOK1 HCP osatengera kuyeretsedwa.

Kukonzekera kwa reagent

1.PBST 0.05%

Tengani 15 ml ya 20×PBST 0.05%, kuchepetsedwa mu ddH₂O, mpaka 300 ml.

2.1.0% BSA

Tengani 1g ya BSA mu botolo ndikusungunula mu 100 ml ya PBST 0.05%, sakanizani bwino mpaka kusungunuka kwathunthu, ndikusunga pa 2-8 ° C.The dilution buffer yokonzedwa ndi yovomerezeka kwa masiku 7.Ndikoyenera kukonzekera pakufunika.

3.Njira yothetsera 2μg/mL

Tengani 48μL ya 0.5 mg/mL Anti-CHO HCP-HRP ndikuchepetsa mu 11,952μL ya 1% BSA kuti mupeze ndende yomaliza ya 2μg/mL yozindikira.

4.QC ndi Kukonzekera kwa CHOK1 HCP Miyezo

Chubu Ayi.	Choyambirira dolution	Kukhazikika ng/ml	Voliyumu μL	1% BSA Voliyumu μL	Voliyumu yonse μL	Chomaliza kuganizira ng/ml
A	Standard	0.5mg/mL	10	490	500	10,000
B	A	10,000	50	450	500	1,000
S1	B	1.000	50	450	500	100
S2	S1	100	300	100	400	75
S3	S2	75	200	175	375	40
S4	S3	40	150	350	500	12
S5	S4	12	200	200	400	6
S6	S5	6	200	200	400	3
NC	NA	NA	NA	200	200	0
QC	S1	100	50	200	250	20

Table: Kukonzekera kwa QC ndi Miyezo

Njira Yoyesera

1.Konzani ma reagents monga zasonyezedwa mu "Reagent Preparation" pamwambapa.

2.Tengani 50μL ya miyezo, zitsanzo ndi QCs (onani Table 3) mu chitsime chilichonse, kenaka yikani 100μL ya Kuzindikira njira (2μg/mL);Phimbani mbale ndi chosindikizira, ndikuyika mbale ya ELISA pa thermomixer.Yalirani pa 500rpm, 25±3℃ kwa 2 hours.

3.Sinthani microplate mu sinki ndikutaya njira yokutira.Pipette 300μL wa PBST 0.05% mu chitsime chilichonse kutsuka mbale ya ELISA ndikutaya yankho, ndikubwereza kuchapa katatu.Sinthani mbale pa chopukutira choyera cha pepala ndikuwuma.

4.Onjezani 100μL ya gawo laling'ono la TMB (onani Table 1) pachitsime chilichonse, sindikizani mbale ya ELISA, ndipo muyimire mumdima pa 25±3℃ kwa 15 min.

5.Pipette 100μL ya njira yoyimitsa mu chitsime chilichonse.

6.Yezerani kuyamwa kwa kutalika kwa 450/650nm ndi owerenga ma microplate.

7.Unikani deta ndi SoftMax kapena mapulogalamu ofanana.Konzani makongoletsedwe okhazikika pogwiritsa ntchito mawonekedwe a magawo anayi.

Standard Curve Chitsanzo

ZINDIKIRANI: Ngati kuchuluka kwa HCP pachitsanzo kupyola malire apamwamba a curve wamba, kumafunika kuchepetsedwa moyenera ndi dilution buffer musanayesedwe.

MFUNDO

Njira yoyimitsa ndi 2M sulfuric acid, chonde gwirani mosamala kuti musamenye!