Rnase Inhibitor (Glycerol wopanda)
Murine RNase inhibitor ndi recombinant murine RNase inhibitor yowonetsedwa ndikuyeretsedwa kuchokera ku E.coli.Imamangiriza ku RNase A, B kapena C mu chiŵerengero cha 1: 1 kupyolera mu mgwirizano wosagwirizanitsa, motero imalepheretsa ntchito ya ma enzyme atatu ndikuteteza RNA kuti isawonongeke.Komabe, sizothandiza motsutsana ndi RNase 1, RNase T1, S1 Nuclease, RNase H kapena RNase kuchokera ku Aspergillus.Murine RNase inhibitor inayesedwa ndi RT-PCR, RT-qPCR ndi IVT mRNA, ndipo inali yogwirizana ndi malonda osiyanasiyana a Reverse transcriptases, DNA polymerases ndi RNA polymerases.
Poyerekeza ndi anthu a RNase inhibitors, murine RNase inhibitor ilibe ma cysteine awiri omwe amakhudzidwa kwambiri ndi okosijeni omwe amachititsa kuti inhibitor iwonongeke.Izi zikupangitsa kuti ikhale yokhazikika pamagawo otsika a DTT (osakwana 1 mM).Izi zimapangitsa kuti zikhale zoyenera kugwiritsidwa ntchito potengera kuchuluka kwa DTT komwe kumatsutsana ndi zomwe zimachitika (mwachitsanzo Real-time RT-PCR).
Akupempha
Izi zitha kugwiritsidwa ntchito kwambiri pakuyesa kulikonse komwe kusokoneza kwa RNase kotheka kupewa kuwonongeka kwa RNA, monga:
1.First-strand cDNA synthesis, RT-PCR, RT-qPCR, etc.;
2.Tetezani RNA kuti isawonongeke mu in-vitro transcription/translation (monga viral replication in vitro);
3.Kuletsa ntchito ya RNase panthawi ya kudzipatula kwa RNA ndi kuyeretsedwa.
Zosungirako
Sungani pa -25～-15 ℃;
Kuzizira kozizira ≤ kasanu;
Itha zaka 1.
Tanthauzo la unit
Chigawo chimodzi chimatanthauzidwa ngati kuchuluka kwa RNase Inhibitor yofunikira kuletsa ntchito ya 5 ng ya RNase A ndi 50%.
Kulemera kwa maselo
RNase Inhibitor (Glycerol-free) ndi mapuloteni a 50 kDa.
Kuwongolera khalidwe
Exonuclease Zochita:
Kuphatikizidwa kwa 40 U ya enzyme ndi 1 μg λ-Hind III digest DNA kwa maola 16 pa 37 ° C sikunapangitse kuwonongeka kwa DNA komwe kumatsimikiziridwa ndi gel electrophoresis.
Endonuclease ntchito:
Kuphatikizika kwa 40 U ya enzyme ndi 1 μg λ DNA kwa maola 16 pa 37 ° C sikunapangitse kuwonongeka kwa DNA komwe kumatsimikiziridwa ndi gel electrophoresis.
Kuyimba Zochita:
Kuphatikizika kwa 40 U ya enzyme ndi 1 μg pBR322 kwa maola 16 pa 37 ℃ sikunapangitse kuwonongeka kwa DNA komwe kumatsimikiziridwa ndi gel electrophoresis.
RNase Zochita:
Kuphatikizika kwa 40 U ya enzyme ndi 1.6 μg MS2 RNA kwa maola 4 pa 37 ℃ kunapangitsa kuti pasakhale kuwonongeka kwa RNA komwe kumatsimikiziridwa ndi gel electrophoresis.
E.coli DNA:
40 U ya Enzyme imadziwika ndi TaqMan qPCR.DNA ya E.coli ndi ≤ 0. 1pg/40U.
Notis
1.Musagwedeze kapena kugwedeza mwamphamvu kuti ma enzyme asamagwire ntchito.
2.RNase inhibitor imagwira ntchito pa kutentha kuyambira 25 ℃ mpaka 55 ℃ ndipo imatsekedwa pa ≥65 ℃.
3.Zochita za RNase H, RNase 1, ndi RNase T1 sizoletsedwa.
4. The pH range for inhibiting RNase activity is wide (yogwira pa pH 5-9), kusonyeza ntchito yaikulu pa pH 7-8.
